The effects of cytotoxic concentration of testosterone, progesterone and estradiol on anti-metastasis CD82/KAI1 expression level in brain glioblastoma cancer cells

Background: Studies have shown that sexual steroids can affect metastasis in cancer cells of nervous system at cellular and molecular level; however, the cellular and molecular mechanism of sexual steroids action on metastasis in cancer cells of nervous system is unclear in many cases. This study aimed at investigating the effects of cytotoxic dose of testosterone, progesterone and estradiol on anti-metastasis CD82/KAI1 genes expression levels in brain glioblastoma cells (A172). Materials and Methods: In this laboratory-experimental study, A172 cells were obtained from Pasteur institute and divided into control group and groups exposed to cytotoxic dose of testosterone, progesterone, and estradiol. Real time PCR also was used to evaluate CD82/KAI1 genes expression levels. The data were statistically analyzed between groups using ANOVA. Results: CD82/KAI1 genes expression level significantly decreased in A172 cells exposed to cytotoxic dose of testosterone, progesterone and estradiol (P<0.001, P<0.05 and P<0.001, respectively). Conclusion: The cytotoxic dose of testosterone, progesterone and estradiol can decrease the anti-metastatic CD82/KAI1 gene expression level and therefore may increase metastasis potential in brain cancer cells.