The effects of cinnamaldehyde and eugenol on human adipose-derived mesenchymal stem cells viability, growth and differentiation: a cheminformatics and in vitro study
Authors
Abstract:
Objective: The aim of this study was to estimate the cheminformatics and qualitative structure-activity relationship (QSAR) of cinnamaldehyde and eugenol. The effects of cinnamaldehyde and eugenol on the viability, doubling time and adipogenic or osteogenic differentiations of human adipose-derived mesenchymal stem cells (hASCs) were also investigated. Materials and Methods: QSAR and toxicity indices of cinnamaldehyde and eugenol were evaluated using cheminformatics tools including Toxtree and Toxicity Estimation Software Tool (T.E.S.T) and molinspiration server. Besides, their effects on the hASCs viability, doubling time and differentiation to adipogenic or osteogenic lineages were evaluated. Results: Cinnamaldehyde is predicted to be more lipophilic and less toxic than eugenol. Both phytochemicals may be developmental toxicants. They probably undergo hydroxylation and epoxidation reactions by cytochrome-P450. The 2.5 µM/ml cinnamaldehyde and 0.1 µg/ml eugenol did not influence hASCs viability following 72 hr of treatment. But higher concentrations of these phytochemicals insignificantly increased hASCs doubling time till 96 hr, except 1 µg/ml eugenol for which the increase was significant. Only low concentrations of both phytochemicals were tested for their effects on the hASCs differentiation. The 2.5 µM/ml cinnamaldehyde and 0.1 µg/ml eugenol enhanced the osteogenesis and decreased the adipogenesis of hASCs meaningfully. Conclusion: According to the cheminformatics analysis and in vitro study, cinnamaldehyde and eugenol are biocompatible and low toxic for hASCs. Both phytochemicals may be suitable for regenerative medicine and tissue engineering when used at low concentrations, but maybe useful for neoplastic growth inhibition when used at high concentrations.
similar resources
the effects of cinnamaldehyde and eugenol on human adipose-derived mesenchymal stem cells viability, growth and differentiation: a cheminformatics and in vitro study
objective: the aim of this study was to estimate the cheminformatics and qualitative structure-activity relationship (qsar) of cinnamaldehyde and eugenol. the effects of cinnamaldehyde and eugenol on the viability, doubling time and adipogenic or osteogenic differentiations of human adipose-derived mesenchymal stem cells (hascs) were also investigated. materials and methods: qsar and toxicity ...
full textDifferentiation of Mesenchymal Stem Cells Derived From Human Adipose Tissue into Cholinergic-like Cells: In Vitro Study
Introduction: Cholinergic-associated diseases currently constitute a significant cause of neurological and neurodegenerative disabilities. As the drugs are not efficient in improving the suffered tissues, stem cell treatment is considered an effective strategy for substituting the lost cells. Methods: In the current study, we set out to investigate the differentiation properties of human adip...
full textComparison of the effects of extremely low-frequency Electromagnetic field and Betaine on in vitro osteogenic differentiation of human adipose tissue derived-mesenchymal stem cells
Background & Aim: Extremely low-frequency electromagnetic field (ELF-EMF) and betaine are safe factors in bone fracture repair. This study aimed to compare the effects of these two stimuli on osteogenic differentiation of human adipose stem cells (hADSCs). Methods: After obtaining written informed consent, cells were extracted from abdominal adipose tissue and then cultured in vitro until the ...
full textHuman Mesenchymal Stem Cells Derived from Adiopose Tissue and Placenta and the Adipocytic and Osteocytic Differentiation
Introduction: Mesenchymal stem cells can be isolated from adult tissues, such as the adipose tissue, or other sources. Among all these sources, adipose tissue because of easy access, and placenta due to its immunomodulatory properties, in addition to another useful properties, were attracted more attention to themselves. Isolation and comparing these two different sources can help us for acces...
full textDifferentiation of adipocytes and osteocytes from human adipose and placental mesenchymal stem cells
Objective(s):Mesenchymal stem cells (MSC) can be isolated from adult tissues such as adipose tissue and other sources. Among these sources, adipose tissue (because of easy access) and placenta (due to its immunomodulatory properties, in addition to other useful properties), have attracted more attention in terms of research. The isolation and comparison of MSC from these two sources provides a ...
full textMy Resources
Journal title
volume 6 issue 6
pages 643- 657
publication date 2016-11-01
By following a journal you will be notified via email when a new issue of this journal is published.
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023