The Effect of Iron on Malignant Lymphoblastic Cells Survival and Its Mechanism

Authors

  • Abedi, Marjan Department of Biological Sciences and Microbiology, School of Biological Sciences and Technologies, University of Isfahan
  • Esmaeili, Abolghasem Department of Biological Sciences and Microbiology, School of Biological Sciences and Technologies, University of Isfahan
  • Rahgozar, Soheila Department of Biological Sciences and Microbiology, School of Biological Sciences and Technologies, University of Isfahan
Abstract:

Background and Objectives:Anemia is a common complication of chemotherapy. In order to resolve this problem, multiple red blood cell transfusions are administered, leading to iron overload. Given the confirmation of positive correlation between the increased bone marrow iron stores and adverse response to the treatment in the previous study, the effect of iron on the proliferation of acute lymphoblastic leukemia (ALL) cell lines cell lines and its underlying mechanism were investigated in the current study.   Methods:Nalm6 and CCRF-CEM cell lines were selected as representatives of B-ALL and T-ALL Immunophenotypes and were treated with different concentrations of holo-transferrin (1 - 120 µM) and ferric ammonium citrate (FAC) (400 - 25000 µM). The cellular iron uptake was confirmed by AAS test. The cell proliferation and levels of intracellular free radicals (ROS), were evaluated by MTT and flow cytometry, respectively. Statistical analysis was performed using GraphPad prism software by one-way ANOVA test.   Results: The effect of holo-transferrinon cell proliferation was not significant. However, FAC enhanced the proliferation rate of both ALL cell lines over 50%. Evidence showed that iron induced intracellular ROS, so that FAC in the concentration of 400 µM may induce the intracellular ROS over 50% (55.27 6 6.36% vs. 100%, p < 0.001).   Conclusion: Iron can support these cancerous cells by inducing ROS and augmenting leukemic cell proliferation. Therefore, the present study suggests that the volume of the injected blood in ALL patients should be minimized to prevent iron overload

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

an investigation of the impact of self monitoring on langauge teachers motivational practice and its effect on learners motivation

the central purpose of this study was to conduct a case study about the role of self monitoring in teacher’s use of motivational strategies. furthermore it focused on how these strategies affected students’ motivational behavior. although many studies have been done to investigate teachers’ motivational strategies use (cheng & d?rnyei, 2007; d?rnyei & csizer, 1998; green, 2001, guilloteaux & d?...

the effect of functional/notional approach on the proficiency level of efl learners and its evaluation through functional test

in fact, this study focused on the following questions: 1. is there any difference between the effect of functional/notional approach and the structural approaches to language teaching on the proficiency test of efl learners? 2. can a rather innovative language test referred to as "functional test" ge devised so so to measure the proficiency test of efl learners, and thus be as much reliable an...

15 صفحه اول

the effect of task complexity on lexical complexity and grammatical accuracy of efl learners’ argumentative writing

بر اساس فرضیه شناخت رابینسون (2001 و 2003 و 2005) و مدل ظرفیت توجه محدود اسکهان (1998)، این تحقیق تاثیر پیچیدگی تکلیف را بر پیچیدگی واژگان و صحت گرامری نوشتار مباحثه ای 60 نفر از دانشجویان زبان انگلیسی بررسی کرد. میزان پیچیدگی تکلیف از طریق فاکتورهای پراکندگی-منابع تعیین شد. همه ی شرکت کنندگان به صورت نیمه تصادفی به یکی از سه گروه: (1) گروه موضوع، (2) گروه موضوع + اندیشه و (3) گروه موضوع + اندی...

15 صفحه اول

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 13  issue 11

pages  73- 83

publication date 2020-02

By following a journal you will be notified via email when a new issue of this journal is published.

Keywords

No Keywords

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023