The Effect of Interval Training and Resveratrol on Expression of ‎PGC-1α and PDK4 Genes in Gastrocnemius Muscle Tissue of Elderly ‎Type 2 Diabetic Rats‎

Aims: The purpose of this study was to determine the effect of exercise training with resveratrol on PGC-1α and PDK4 gene expression in gastrocnemius muscle tissue of elderly type 2 diabetic rats. Materials and Methods: 42 male rats with type 2 diabetes, 250 to 300 g were divided into 6 groups; healthy-control, diabetic-control, diabetic+interval exercise, Diabetic+Supplement, Diabetic+interval exercise+ Supplement and Saline. The exercise included 10 sets of one-minute activity with 50% intensity and 2 minutes of rest between sets, and each week increased by 2 m per minute. The daily 20 mg/kg body weight Resveratrol supplementation was injected to rats. Gene expression PDK4 and PGC-1α in the muscle gastrocnemius were measured by RT-PCR method. Findings: The results showed that the highest expression of PDK4 and PGC-1α gene in gastrocnemius muscle was observed in supplementation+interval exercise and lowest levels in patient and saline groups. Conclusion: The results showed that the combination of resveratrol and interval exercise can have beneficial effects on PDK4 and PGC-1α gene expression in gastrocnemius muscle of elderly with type 2 diabetes mice and ultimately reduce the risks of diabetes complications.