The effect of eight weeks of endurance training and olive oil supplementation on IL-1ß and NLRP3 indices in obese male rats

Background: Obesity is the biggest public health challenge of the current century, and the health departments of most countries in the world are involved in the problems and complications of increasing obesity and identifying the mechanisms involved. Therefore, the aim of this study was to investigate the effects of two months aerobic training and olive extract supplementation on IL-1β and NLRP3 in obese male rats. Materials and Methods: In experimental research, twenty-four male rats with two months of age were fed high fat diet for 8 weeks to reach a mean weight of 320±30. Then they were randomly divided into four groups (control group, endurance training group, olive oil supplementation group and endurance training and olive oil supplementation group). Olive oil supplementation (Oos) and aerobic training (AE) (at 80% of VO2max) were conducted for two months. The IL-1β and NLRP3 levels were measured by ELISA method. The data were analyzed using two-way ANOVA at 95% confidence level. Results: Both AE and Oos and their combination reduced IL-1β levels with greater changes observed in both training groups (P<0.05). The NLRP3 was only decreased in the combination of AE and Oos groups and the Oos and ET were failed to change this factor level. Conclusion: Exercise training and Olive oil consumption could be beneficial in lowering inflammation in obese subject, however; more research remains to be done because of the lack of similar evidence and also limitations in the study.