THE EFFECT OF AEROBIC TRAINING ON MUSCLE ANGIOGENESIS AND DOWNSTREAM FACTORS OF PI3KR2 PATHWAY IN CARDIAC TISSUE OF DIABETIC RATS

Background & Aims: Regarding the effect of diabetes on vascularization processes and since the beneficial effects of aerobic exercise on the cardiovascular system have been proven, therefore, the aim of this study was to determine the effect of aerobic exercise on muscle angiogenesis and downstream factors of the PI3KR2 pathway in cardiac tissue of diabetic rats. Materials & Methods: Twenty diabetic male Wistar rats (mean weight, 191.9±10.85) were divided into two groups of control (n=10) and training (n=10) and the groups were matched based on weight. 48 hours after the last training session, cardiac tissue samples were taken after an overnight fast. Immunohistochemistry (alkaline phosphatase activity) was used to measure the cardiac muscle capillary density. Also, measuring AKT and eNOS proteins were measured by ELISA method. Results: Independent t-test showed that the 8-week aerobic training significantly increased the capillary density (p=0.018) and also caused a significant increase in the expression of AKT and eNOS proteins as compared to control group (p=0.001). Conclusion: The results of this study indicate the development of angiogenesis by aerobic exercise in diabetic conditions. According to the results of this study, it can be expressed that aerobic exercise can be used as a non-drug treatment to improve heart perfusion in diabetic patients.