The Effect of Aerobic Training and Adenosine on the Expression of SREBP-1C and A1 Receptor in Hepatic Fat-fed Rats

Background and Objectives: Few studies have examined so far the effect of adenosine receptors’ injection and its downstream pathway on the liver’s fat metabolism. The aim of this study was to investigate the type of aerobic exercise and adenosine on the expression of sterol regulatory binding protein 1c SREBP-1c and the adenosine receptor A1 in the liver in the rats fed with high-fat foods. Materials and Methods: Forty rats were randomly divided into eight groups: control of standard diet, high fat diet, high fat diet and placebo, high fat diet and Adenosine injection, high fat diet+high-intensity interval training (HIIT) and adenosine injection, high fat diet+HIIT and placebo, high fat diet+aerobic training and adenosine injection, high fat diet+aerobic training, and placebo. The subjects received 13 weeks of high-fat diet in the first stage. In the second stage, they trained for 12 weeks each week for 5 sessions. An analysis of one-way independent variance was used to analyze the data in the diet groups, and two-way analysis of variance was used to determine the effect of exercise interaction and adenosine. Real time PCR was used to determine the expression of genes. Results: SREBP1-c and A1 gene increased significantly in fatty diet (P = 0.016, and P = 0.019, respectively), HIIT and aerobic training had a significant effect on the reduction of SREBP1-c and A1 gene expression (P = 0.003, and P = 0.001, respectively). SREBP1-c and A1 in the adenosine injection group had a significant decrease (P = 0.004, and P = 0.002, respectively). The interaction of endurance training-adenosine had a significant decrease in the expression of A1 (P = 0.004) comparing to HIIT-Adenosine training (P = 0.019). Conclusion: Regardless of the intensity of exercise as an effective factor in the expression of lipogenesis genes, the adenosine dosage with increasing or decreasing its receptor (A1) following consumption of a high fat diet may have a significant role in the lipogenic expression of genes.