The Effect of a Course of Aerobic Exercise With Hypoallergenic Supplement of Tribulus Terrestris on Mitochondrial Oxidative Stress Markers of Cardiac Tissue in Rats Toxicated With Hydrogen Peroxide

Introduction: Oxidative stress causes DNA damage and apoptosis and leads to cardiovascular disease. The aim of this study was to evaluate the effect of a course of aerobic exercise with hypoallergenic supplement of Tribulus terrestris on mitochondrial oxidative stress markers of cardiac tissue in rats toxicated with hydrogen peroxide. Method: To implementation of this experimental research, 42 male wistar rats randomly were divided into 7 groups include 1) Control (toxicated with hydrogen peroxide without extract), 2) Aerobic exercise, 3) Aerobic exercise - Tribulus terrestris extract 5mg/kg, 4) Aerobic exercise - Tribulus terrestris extract 10mg/kg, 5) Tribulus terrestris extract 5mg/kg, 6) Tribulus terrestris extract 10mg/kg, 7) Healthy control. All groups received 100 mg/kg body weight of hydrogen peroxide for 14 days intraperitoneal. Rats in supplemented groups received Tribulus terrestris hydroalcoholic extract with doses of 5 and 10 mg per day by gavage method. Aerobic training was performed on a treadmill at a speed of 23 m/min, 30 min/day, 5 days/week for eight weeks. 24 hours after the last training session, rat heart tissue was collected. Data were analyzed by Independent t-test, two-way ANOVA and Bonferroni post hoc test at the P<0.05.  Results: Tribulus terrestris extract and mixed intervention of tribulus terrestris extract with aerobic training led to significant increase in levels of O-6-methyl guanine-DNA-methyl transferase, Prooxidants-antioxidant balance (PAB) and Cytochrome C Oxidase, as well as significant decrease in adenosine triphosphate (ATP) and Malondialdehyde (MDA) the heart tissue of rats compared to the control group poisoned with hydrogen peroxide (P<0.05). Conclusion: It seems that iintervention of regular aerobic training and the various doses of tribulus terrestris extract is a moderating factor in mitochondrial biogenesis and is effective in reducing the DNA damage in heart tissue and the lower dose has more benefits.