The effect of 8 weeks of HIIT training and supplementation of black grape seed extract on Wnt and Β-catenin gene expression in pancreatic tissue in male rats with type 2 diabetes

Objective:The aim of the present study was to investigate the effect of 8 weeks of HIIT training and supplementation of black grape seed extract on Wnt and Β-catenin gene expression in pancreatic tissue in male rats with type 2 diabetes. Methods:In this experimental study, 40 male Wistar rats, with average weight of 250 ± 20 gr, after induction of diabetes by STZ, were randomly divided into 5 groups: training- supplement, training-placebo ,supplement and diabetic-control and baseline. After becoming diabetic, rats were familiarized with protocol for one week and then trained for eight weeks,five days a week. They ran on treadmill with 90% of VO2 max. Intragastric injection of GSPE extracted from black grape seed (Cabernet sauvignon) once a day for 8 weeks (250 mg/kg, proanthocyanidin content >96%.( 24 hours after the last session of training, pancreas tissue was extracted, and the level of gene expression was measured by Real Time-PCR and scale ( ).In all statistical analyzes, the statistical significance level was considered to be 0.05. Results: eight weeks and supplement significantly increased the mean expression of Wnt and Β-catenin genes in pancreatic tissue of type 2 diabetic rats but the effect of exercise with black grape seed extract on the expression of Wnt genes in the pancreas of diabetic rats was not significant. Conclusion:It seems that regulating the expression of Wnt and Β-catenin genes through exercise and consumption of black grape seed extract is likely to improve and maintain the function of pancreatic beta cells, especially in diabetic samples.