The Effect of 6 Weeks Aerobic Training and Aqueous Extract of Caraway Seed (Carum Carvi) on the Expression of CTRP12 Gene, Body Weight Changes and Subcutaneous Adipose Tissue in Obese Male Rats: An Experimental Study

Background and Objectives: Caraway (Carum carvi) is one of the medicinal plants which is traditionally used for weight loss. The purpose of the present study was to determine the effect of 6 weeks aerobic training and aqueous extract of caraway seed (carum carvi) on expression of CTRP12 gene, body weight changes and subcutaneous adipose tissue in obese male rats. Materials and Methods: In this experimental study, 24 fat male rats were provided a high-fat diet for 12 weeks to induce obesity, following which they were divided randomly into four groups: 1. high-fat control diet, 2. high-fat diet+ exercise, 3. high-fat diet+caraway, 4. high-fat diet+exercise+caraway. The training groups performed aerobic exercise 5 days in week for 6 weeks (approximately 50-70% VO2max). Relative expression of CTRP12 mRNA was measured using real-time polymerase chain reaction (real-time PCR), body weight changes and subcutaneous adipose tissue. Data were analyzed using one-way ANOVA and Tukey’s post hoc test. Results: Relative expression of CTRP12 mRNA significantly increased after 6 weeks of aerobic training and consumption of carum carvi extract in group 4 compared to groups 1 and 3, in group 1 compared to group 2, and animal weight and subcutaneous fat weight in groups 4, 3 and 2 significantly decreased compared to group 1. Conclusions: Considering the results of the present study, it is recommended to use moderate aerobic exercise and carum carvi to reduce the risk of obesity and weight. Key words: Aerobic training, Caraway, CTRP12, Subcutaneous fat, High-fat diet, Rat, Obesity Funding: This study was funded by Sedigheh Taherzadeh. Conflict of interest: None declared. Ethical approval: The Ethics Committee of the Sport Sciences Research Institute of Iran approved the study (IR.SSRI.REC.1397.395). How to cite this article: Taherzadeh S, Mogharnasi M, Rasoulian B, Kaeidi A, Khosravi A. The Effect of 6 Weeks Aerobic Training and Aqueous Extract of Caraway Seed (Carum Carvi) on the Expression of CTRP12 Gene, Body Weight Changes and Subcutaneous Adipose Tissue in Obese Male Rats: An Experimental Study. J Rafsanjan Univ Med Sci 2020; 19 (1): 39-52. [Farsi]