Synthesis and Characterization of Novel Modified and Functionalized Silica Nano-particles for Protein Delivery Applications

In this study, the synthesis, characterization and controlled release behavior of new Hollow Silica Nano particles (HSNPs) and Magnetic Silica Nano Particles (MSNPs) were studied. Magnetic Silica Nano particles (MSNPs), as drug delivery vehicles, were synthesized through the coating of Fe3O4 nano-crystals with silica layers. The HSNPs were obtained by removal of Fe3O4 templates with hydrochloric acid and then calcinated at the high temperature. In the first step, MSNPs and HSNPs were modified and the amine and aldehyde functional groups were introduced on the silica surfaces. In the next step, positively charged silica nano particles were synthesized through the quaternization of Aminated Silica Nano Particles (ASNP) with sodium monochloroacetate (SMCA). Following characterization, insulin was loaded into the nano particles as a model system which demonstrated a notable sustained drug release. The study shows that the HSNPs could load a higher amount of drug. The release rates from the HSNPs were faster than those of MSNPs.