Synthesis and characterization of ciprofloxacin loaded histidine modified chitosan nanoparticle for eradication of Pseudomonas aeroginosa biofilm

Background: Pseudomonas aeruginosa biofilm is one of the problems in antibiotic treatment of infections. Nanomedicines such as chitosan can carry multiple drugs and improve the therapeutic effects of antibiotics. Objectives: The study aimed to synthesis and characterization of ciprofloxacin loaded chitosan nanoparticle for eradication biofilm of P. aeroginosa. Methods: Cipro-CS microparticles were prepared by ionic gelation method and their size, zeta potential, and drug release pattern were determined. MBEC and MBIC of different groups of antibiotic (Ciprofloxacin, Ciprofloxacin & PAβN, CS Ciprofloxacin, and CS Ciprofloxacin & PAβN) were performed on biofilm samples of P. aeroginosa. Results: Ciprofloxacin loading efficiency was 35.51%, and encapsulation efficiency was 55.06%. Released ciprofloxacin from CS nanoparticles was %80 after 24 hours. Biofilm production was positive in %96.7 of the isolates while %30.1 of the samples had strong biofilm. The best result for MBIC was CS Ciprofloxacin, CS Ciprofloxacin & PAβN, Ciprofloxacin & PAβN, and Ciprofloxacin respectively. For MBEC the result was slightly different and from the best to better  CS Ciprofloxacin & PAβN, CS Ciprofloxacin, Ciprofloxacin & PAβN, and Ciprofloxacin. Conclusion: Today, with increasing antibiotic resistance, there are many challenges in treating infections. Due to the role of biofilm in antibiotic resistance, researchers are looking for new antibiotics to treat infections.