PCR-mediated identification of Methicillin and Vancomycin resistant genes in Staphylococcus aureus strains isolated from the nasal cavity

Authors

  • Fatemeh Mahdavi Niaki Department of Cellular and Molecular Biology, Islamic Azad University, Babol Branch, Babol, Iran.
  • Majid Alipour Department of Cellular and Molecular Biology, Iran. Islamic Azad University, Babol Branch, Babol, Iran.
  • Yousef Yahyapour Infectious Diseases & Tropical Medicine Research Center, Babol University of Medical Sciences, Babol, Iran
  • Zahra Mahdavi Niaki Department of Cellular and Molecular Biology, Islamic Azad University, Babol Branch, Babol, Iran.
Abstract:

Staphylococcus aureus is colonized in the human nasal cavity and would contaminate hospital and therapeutic environments. This bacterium has a genetic diversity in terms of resistance to antimicrobial agents. Therefore, the purpose of this study was identificatied of Methicillin and Vancomycin resistant genes in Staphylococcus aureus strains which has been isolated from the nasal cavity. 189 patients referred to Amol city health center were sampled. Staphylococcus aureus identification was performed by standard methods. Resistance to antibiotics of Vancomycin, Methicillin, Cefpime, Ceftriaxone, Cefixime, nalidixic acid, Cefazolin, Stiffenoxime, Cefotaxime, Ceftazidime, Imipenem and Ciprofloxacin were identified by disk diffusion method and recommendations of the Laboratory and Clinical Standardization Organization (CLSI). Polymerase chain reaction was performed to detect mecA, vanA and femB genes. 32.2% of the patients were carriers of Staphylococcus aureus in their nose. In this research, 68.85% of the isolates were resistant to Methicillin. 100% resistance to Cefixime and Nalidixic acid antibiotics was observed. Subsequently, the highest amounts of resistance belonged to Cefazolin (96.72%) and Cefepime (86.88%). Resistance to Vancomycin was 70.49%. The lowest amounts of resistance were observed in the two antibiotics Ciprofloxacin and Imipenem, with incidences of 6.55% and 9.83% in isolates, respectively. Genetically, no resistance was observed for Vancomycin and none of the tested isolates were carried vanA gene. Of 61 separated Staphylococcus aureus, 77.04% of them carried mecA gene and 90.16% of them had femB gene. This study showed that the main resistance to Penicillin, Oxacillin and Methicillin in Amol city is due to mecA and femB genes.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

Prevalence of Vancomycin Resistance in Methicillin-Resistant Staphylococcus Aureus

Background & Aims: Staphylococcus aureus is one of the most common pathogens in nosocomial infections. Vancomycin is the most important therapeutic drug of choice for treatment of infections caused by methicillin-resistant Staphylococcus aureus (MRSA) strains. Therefore, vancomycin-resistant Staphylococcus aureus (VRSA) or vancomycin-intermediate Staphylococcus aureus (VISA) strains are warning...

full text

Screening of Methicillin and Vancomycin resistant Staphylococcus aureus in the nasal of hospital personnel of Khorram Abad ,Iran

Abstract Background and Objectives associated infections has been gained attention by most researchers. We designed this cross-sectional study to evaluate MRSA and VRSA frequency in the Shohadai Ashayer hospital of Khorram Abad, Iran. : Increased drug resistance, and its Material and Methods: moistened with normal saline from interior nares of 300 personnel and immediatel...

full text

Survey of the Lethal Effect of Ciprofloxacin and Supernatant Isolated from Staphylococcus Aureus under the Stress of Ciprofloxacin on Methicillin-Resistant Staphylococcus Aureus Strains Isolated from Clinical Specimens

Background and Aim: Staphylococcus aureus is gram-positive coccus that is one of the most important causes of nosocomial infections and cause cutaneous or subcutaneous infections. Among these bacteria Methicillin-resistant Staphylococcus aureus (MRSA) are the most important. The aim of the present study was to investigate the lethal effect of a substance isolated from Staphylococcus aureus unde...

full text

Genotypic Investigation of Methicillin Resistant Staphylococcus aureus Strains Isolated from Urinary Tract Infection

Background and purpose: Methicillin resistant Staphylococcus aureus (MRSA) have recently emerged as major causes of urinary tract infection (UTI). The aims of this study were to determine antibiotic susceptibility of S. aureus isolated from UTI and to detect the presence of mecA (causing resistance to methicillin) and SCCmec types. Materials and methods: In this cross sectional study, 44 S. au...

full text

genotyping of coa and aroa genes of methicillin-resistant staphylococcus aureus strains isolated from nasal samples in western iran

conclusions methicillin-resistant staphylococcus aureus is an increasingly common cause of nosocomial infections. our results are in agreement with those of other studies reporting that a few specialized clones are responsible for most cases of mrsa nasal carriage. in this study, mrsa strains isolated from different wards of hospital were closely related when analyzed by coagulase gene typing. ...

full text

Methicillin-Resistant Staphylococcus aureus (MRSA), Vancomycin-Resistant Staphylococcus aureus (VRSA), and Vancomycin-Resistant Enterococci‌ (VRE) contamination of food samples in Iran: A systematic review and meta-analysis

During the last years, antimicrobial resistance has become one of the greatest challenges in clinical settings. Staphylococcus aureus and Enterococcus are important nosocomial pathogens worldwide. In different corners of the world, methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant enterococci (VRE) have been isolated from various sources including different foods of an...

full text

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 7  issue 2

pages  896- 902

publication date 2017-12-01

By following a journal you will be notified via email when a new issue of this journal is published.

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023