P-73: Effect of Sodium Dodecyl Sulfate and Different Levels of Clove Bud Extract on Motility, Viability and Plasma Membrane Integrity of Ram Spermatozoa during Cooling and Cryopreservation

Background: Hen egg yolk is a common component of most semen cryopreservation extenders. However, diluents containing the egg yolk may have adverse effects on the quality of the semen. Egg yolk could be solubilized by the addition of sodium dodecyl sulphate (SDS) in semen extenders. Many studies have shown beneficial effects of antioxidants addition to semen freezing extenders. Antioxidants are absolutely critical for maintaining optimal cellular and systemic health. The clove bud oil has been powerful antioxidant properties. However, few studies deal with scavenging effect of clove buds extract in sperm extenders. Materials and Methods: The semen samples were collected from 3 Iranian ram (3-4 years old and 105 kg BW) using artificial vagina, twice a week. Antioxidant property of the ethanol extract of clove bus was determined by DPPH test and then it was added to sperm diluents with concentrations of 0, 35, 75, and 115 mg/ml. The SDS was added at levels of 0 and 0.5 mg per ml of egg yolk. The sperm motility, viability and plasma membrane integrity were evaluated after semen collection, before sperm freezing and after thawing. Results: This study revealed that extenders with SDS was more effective in preservation of motility, viability and integrity of the plasma membrane of spermatozoa (p<0.05). Different levels of clove bud extract affect the sperm characteristics before freezing and after thawing (p<0.05). The best level for clove bud extract was 75 mg/ ml in semen diluents. Conclusion: Therefore we can conclude that adding SDS and plant extract may benefit effects on sperm cryopreservation.