P-7: Testosterone and Gonadotrophin Hormones Plasma Concentration-Induced Changes in Sertoli and Leydig Cells Number in Hyperprolactinemic Mature Male Mice

Background: Hyperprolactinemia is a particular concern in the treatment of schizophrenic patients. The side effects associated with high prolactin levels can have a negative impact on GnRH pulsatile secretion and, consequently LH, FSH and testosterone pulsatility and follows by, on special testicular cells that have these hormon’s receptors such as sertoli and leydig cells. Quantification the degree of correlation between sexual hormones (LH, FSH, Tes) biosynthesis and leydig and sertoli cells number in hyperprolactinemic mature male mice is the aim of this research. Materials and Methods: 18 mature male mice were divided in 2 groups as test and control. For hyperprolactinemia induction, test group received 40 mg/kg sulpiride solution, daily for 45 days IP. Prolactin, LH, FSH and testosterone were measured in the plasma samples of all mice. Testicular tissue samples were fixed, subsequently embedded in paraffin and sections were stained with iron-weigert staining method for assessment of sertoli and leydig cells number. Results: Higher prolactin and lower LH, FSH and testosterone plasma concentration in treated group than control group were found. Sertoli cells number decrease obviously in hyperprolactinemic group in compare with other group. Compensatory increase of leydig cells/mm2 of test group testicular interstitial tissue were observed. Conclusion: Results show that high prolactin serum concentration by impacts on gonadotrophin hormones secretion, causes changes in male germ-cells supporting cells (sertoli) and male sex hormone producing cells (leydig) number that induce secondary infertility in hyperprolactinemic patients.