P-4: Effect of Reduced FSH Serum Concentration Induced by Sulpiride Atypical Antipsychotic Drug on Repopulation Index and Number of Primery Spermatocyte Cells in Adult Male Mice

Background: Usage of some atipycal antipsychotic drugs like sulpiride has severed effects on male fertility by impressions on sexual hormones secretion. These hormones regulate male germ cells proliferation and differentiation. In the present study we sought to elucidate the impact of sulpiride on serum prolactin and FSH concentration. Also effect of FSH on spermatogonium A/B (repopulation index) and primery spermatocyte numbers were evaluated. Materials and Methods: 24 adult male mice were divided in 3 groups as test, control sham and control. Treatment group was injected 40 mg/kg sulpiride solution IP, daily for 45 days. Control sham received placebo. After 45 days all mice were sacrificed by cervical dislocation. Prolactin and FSH in serum samples of all mice were measured. Testicular tissues were fixed in 10% formalin fixative and subsequently embedded in paraffin. Sections were stained with iron-veigert for assessment of spermatogony and primery spermatocyte cells. Results: Outcome shows a significant increase in serum prolactin levels and decrease of FSH serum concentration in treated mice. No significant differences are found between 2 other groups. Repopulation index and the number of spermatocyte cells significantly reduce in treated group in comparison with control sham and control groups. Conclusion: Current study proves that sulpiride antipsychotic drug is able to affect on sperm production by indirect reduction of some sexual hormones serum concentration and can cause secondary infertility in some cases.