P-238: Evaluation of The Effect of Ethanol Consumption on Testis Tissue in Mice

Background: Alcohol affects reproduction in both men and women in a number of ways. Chronic exposure to ethanol may results in pathophysiologic changes in cellular function. The present work was designed to investigate the morphology of testis submitted to experimental ethanol ingestion. Materials and Methods: Twenty adult male mice were divided in 2 groups: control sham (salin normal) and test group received ethanol (3 g/kg body weight as 25%, v/v) was given by gastric intubation daily for 45 days. After 45 days all mice were sacrificed. their testis were immediately removed, washed in normal saline and placed in fixative formalin. After routine histological processing and embedding in paraffin, sections of 5 μm thickness were cut and stained by Hematoxylin-Eosin. Prepared specimens were examined and photographed by light microscopy. Results: Abnormal testicular changes occurred and semeniferous tubules destructions were also observed. The number of leydig cell,spermatogonia cells,sertoli cells were decreased. Conclusion: The present study demonstrates that chronic consumption of ethanol has toxic effect on spermatozoa and structure of testis.