P-21: Protective Effect of Black Grapes Seed Hydroalcholic Extract on Sperm Parameters in Male Mice Treated with Fluoxetine

Background: Fluoxetine (FLX) is an Antidepressant compound. FLX produces undesired side effects including anxiety, sleep disturbances, sexual dysfunction and gastrointestinal disturbances. The grape is a significant source of nutritional antioxidants, such as polyphenols, anthocyanins, flavonids as well as biologically active dietary components. Present study was performed to investigate the protective effect of antioxidant treatment with Black grape seed extract (BGSE) on FLX -induced sperm damage. Materials and Methods: In this study 24 mature male mice were used. The animals divided into four groups as, control, FLX (20 mg/kg), FLX (20 mg/kg) + BGSE (100mg/kg) and BGSE (100 mg/kg) orally for 42 days . The caudae epididymides spermatozoa were obtained on day 42 in all groups. Sperm count, viability, maturity, motility and DNA double-strand breaks were examined. Results: The results revealed that sperm count and sperm motility was significantly (p<0.05) decreased in FLX treated group in compared to other groups. Sperm viability and sperm maturity in FLX group was not significant statically between all groups. Sperm DNA damage increased significantly in FLX group in comparisons with other groups. Conclusion: Fluoxetine can induce male infertility. Simultaneously administriation FLX and BGSE prevent negative effect of FLX on sperm parameters.