P-194: Follicular Fluid Adiponectin Concentrations in Follicles in The Luteal Phase of The Estrous Cycle in Dairy Cows

Background: Adiponectin is an adipocytokines which is secreted from the adipose tissue of human and domestic animals. No information is available regarding the follicular fluid adiponectin concentrations in dairy cows. Therefore, the aim of the present study was to determine the follicular fluid adiponectin concentrations in different-sized follicles in the luteal phase of the estrous cycle in dairy cows. Materials and Methods: Ovaries and blood of 18 Holstein dairy cows in the luteal phase were collected from the Shiraz slaughterhouse. The clear follicles divided into three diameter groups (small, 3-5 mm; medium, 6-9 mm and large, ≥10 mm) and their fluid was aspirated using a needle. Concentrations of follicular fluid adiponectin (using ELISA Cusabio Biotech kit, China) and serum progesterone (using radioimmunoassay Immunotech kit, France) were determined. Differences of means and standard deviations of follicular fluid adiponectin concentrations in three groups were analyzed using one way ANOVA. Correlation coefficient of adiponectin concentration between the follicular fluid of different sizes of follicles and concentrations of serum progesterone was also analyzed using the Spearman&#039;s test (SPSS 11.5, p<0.05). Results: Progesterone assay showed that all cows were in the luteal phase of their estrous cycle. Follicular fluid adiponectin concentrations of small-sized (5.05 ± 0.78 μg/mL), medium-sized (4.89 ± 0.79 μg/mL) and large-sized (5.04 ± 1.27 μg/mL) follicles in luteal phase were not different (p>0.05). In the luteal phase of the estrous cycle, a positive correlation was observed between the adiponectin concentrations in large-sized and medium-sized (r=0.72, p=0.01), large-sized and small-sized (r=0.53, p=0.01), medium-sized and small-sized (r=0.86, p=0.01) follicles. Further, There was a positive correlation between the serum progesterone and follicular fluid adiponectin in the medium-sized follicles (r=0.50, p=0.03). Conclusion: Results of the present study showed that follicular fluid adiponectin concentration does not alter as the follicle development occurs during the luteal phase of the estrous cycle in dairy cows.