P-144: Evaluating The Role of Long-Term Administration of Methamphetamine on Sperm Motility, Acrosome Reaction and Chromatin Packaging

Background: This study investigated the probable effects of daily administration of methamphetamine (MA) on sperm motility, acrosome reaction (AR), acridine orange (AO), sperm chromatin dispersion (SCD) test and chromomycin A3 (CMA3) in mice. Materials and Methods: Twenty four NMRI male mice were administrated three different doses of MA intraperitoneally (n=6 in each group). Different doses of MA were administrated thirty days to different groups as follows: low, medium and high dose, 4, 8 and 16 mg/kg, respectively. Control group was only received saline instead. The mice were weighed before and after MA administration. Sperm motility and AR were analyzed accordingly. The effects of MA on sperm DNA integrity were assessed using AO, SCD and CMA3 tests. Results: There was a significant decrease in body weight in high dose group compared to other groups. Sperm analysis showed that percentage of progressive motility was decreased in medium and high dose groups compared to controls (24.67 ± 6.32, 20.17 ± 4.1 and 46.4 ± 3.8, respectively, p<0.001). A significant increasing level in AR and SCD analysis was seen in high dose group (p<0.001). The results of AO and CMA3 exhibited a significant increase in high and medium groups compared to control group (p<0.0001). Conclusion: It appears that MA may significantly reduce sperm progressive motility. The sperm DNA integrity seems to be affected by MA administration in a dose-dependent manner as well.