P-14: Sperm Quality in Male Adult Rat That Were Treated with Cyclosporine

Background: Spermatozoa are germ cells that have a pivotal role at fertilisation. Infertility is a problem with a large magnitude. Sperm damages are one of the factors that cause infertility. Reactive oxygen species (ROS) like hydrogen peroxide (H2O2), superoxide anion (O2−U) or molecules and/or hydroxyl radical (UOH) affect both male and female gametes. Cyclosporine A (CsA), a neutral lipophilic cyclic undecapeptide (C6H11N1lO12), it is a powerful immunosuppressive agent that has markedly improved graft survival rates in organ transplantation. CsA therapy induces overproduction of reactive oxygen species (ROS) in hepatocytes and lowers their antioxidant capacity. Materials and Methods: In this study 16 adult male Rat, weighing 200 ± 20 g were used.The animals were divided into two groups (control, cyclosporine). CsA were given at the dose of 45 mg/kg/day by gavage for 45 days. After 45 days animals were sacrificed by Dislocation of cervical vertebra and cauda epididymis were mince in 1ml HTF+ 4mg/ml Bovin serom Albumin and for 30 minutes put in CO2 incubator 37°C, After get out sperm in the medium, Different parameters, such as sperm viability, motility, sperm count and sperm DNA fragmentation were measurd. Results: This study demonstrates that CsA have a Profound effect on the reproductive system of the male rats. CsA arrest the spermatogenic process, and the number of spermatids and spermatozoa were decreased. CsA also reduced motility and the fertilitizing ability of spermatozoa . Conclusion: That CsA can cause negative effects on sperm and decreased sperm count and live sperm and increase sperm with damaged DNA and immature sperm.