P-130: Efficacy of Commercial In Vitro Maturation Medium Is Similar to Home-made Medium for In Vitro Maturation of Human Oocytes

Background: In vitro maturation (IVM) of immature oocyte is a promising treatment option for infertile women.IVM procedures attempt to mimic the natural conditions of the follicular environment. The technique of IVM of oocytes has been successful after the first trial using culture of human oocytes in follicular fluid. The crucial part of this technique is the maturation process itself, and the choice of the base medium for human IVM is considered particularly difficult. The aim was to compare the efficacy of two culture media namely, home-made human follicular fluid (HFF) medium and commercial IVM medium in maturation of human oocytes. Materials and Methods: The oocytes were obtained from stimulated ovaries of infertile patients who underwent ICSI.101 immature oocytes and then were cultured in HFF medium containing Ham’s F10 supplemented with LH+FSH, and 85 immature oocytes were cultured in commercial IVM medium supplemented with LH+FSH.They were checked for maturity 24-40 hours after culture at 37°C/ 5% Co2. Finally, the maturation rates were compared between two groups of oocytes. Results: The rate of maturation inHFF medium group was 59.5%, whereas in commercial IVM medium group was 65.8% (p=0.364, OR=0.74, 95%CI=0.40-1.35). The rate of degeneration in HFF and commercial medium group was 3 and 4%, respectively. Conclusion: HFF medium, although widely used for embryo fertilization and maintenance in ART techniques, has similar efficacy as commercial IVM medium for the maturation of human oocytes.