P-127: The Effect of Supplementation of In Vitro Culture Medium with FGF2 on Ovine Embryo Development

Authors

  • Hajian M
  • Hosseini SM
  • Ostadhosseini S
  • Riasi A
Abstract:

Background: Fibroblast growth factors are a great family of growth factors that exert variety of functions in vertebrates including cell growth, survival, differentiation and migration. FGFRs are transmembrane tyrosine kinase receptors transmitting FGF signals. Four types of FGFRs (1/2/3/4) exist in vertebrates. FGF2 is one of the FGFs detected in bovine and ovine uterus flushes, supposing that it has effects on embryo development. Materials and Methods: In vitro produced ovine embryos via IVF were removed at different developmental stages (zygote, 2-4cell, 8-16cell, morula, and blastocyst) from day 1 to day 7 and positioned in RLT for investigation of FGFRs expression pattern by Real- Time PCR. In the case of FGF2 treatment effect, from day1, embryos were divided into two groups (control or 500ng/ml FGF2) in serumfree conditions. FGF2 was present in treatment group medium whole days and developmental rate were assessed during development until day8. Finally, expanded and hatched blastocysts differentially stained and cell numbers recorded by using fluorescence microscope. Results: According to our data all four types of FGFRs evinced dominant expression at morula stage while in the other stages FGFRs expression were in low levels. FGF2 supplementation did not significantly change the blastocyst formation rate and total cell numbers in FGF2 supplemented group compared to the control until day8. even though FGFRs were present in ovine embryos, their expression were in low levels except in morula. It is unclear whether FGFRs are in adequate levels to transduce FGF2 signals in ovine embryos. However, it does not seem that FGF2 exerts any proliferative or developmentally amending effect on ovine embryos up to day 8, according to our data. It is not known whether proliferative respond to FGF2 will appear from hatched blastocyst stage onward, one in which high proliferation rates prevail. Conclusion: We found neither a prominent effect of FGF2 on blastocyst formation rate nor proliferation in ovine embryos up to day 8 post fertilization.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

: the effect of sericin levels (silk glue protein) on rate of in vitro maturation, fertilization and culture of sheep oocytes

هدف از آزمایش اول بررسی اثر سطوح مختلف سریسین [0 (control), 0.1, 0.5, 1.0, 2.5 %] افزوده شده به محیط , ivm بر cumulus cell expansion، بلوغ هسته و توسعه متوالی جنین، در گوسفندان نژاد سنجابی در فصل تولید مثلی می باشد. از سرگیری میوز به وسیله خارج شدن اولین پولار بادی اندازه گیری و هم چنین درصد رسیدن جنین های دو سلولی به مرحله کلیواژ و بلاستوسیت نیز به عنوان نشانه ای از میزان شایستگی توسعه اولیه ج...

the effect of e-64 on the developmental competence of sheep cocs during in vitro maturation

in the present study, the effect of e-64 at different concentrations (0.5, 1, 5 and 10 µm) added to (1) the ivm medium on oocyte nuclear maturation and developmental competence of ovine oocytes, and (2) to the ivc medium on embryonic development of ovine embryos were investigated.

effect of oral presentation on development of l2 learners grammar

this experimental study has been conducted to test the effect of oral presentation on the development of l2 learners grammar. but this oral presentation is not merely a deductive instruction of grammatical points, in this presentation two hypotheses of krashen (input and low filter hypotheses), stevicks viewpoints on grammar explanation and correction and widdowsons opinion on limited use of l1...

15 صفحه اول

the washback effect of discretepoint vs. integrative tests on the retention of content in knowledge tests

در این پایان نامه تاثیر دو نوع تست جزیی نگر و کلی نگر بر به یادسپاری محتوا ارزیابی شده که نتایج نشان دهندهکارایی تستهای کلی نگر بیشتر از سایر آزمونها است

15 صفحه اول

the effect of taftan pozzolan on the compressive strength of concrete in the environmental conditions of oman sea (chabahar port)

cement is an essential ingredient in the concrete buildings. for production of cement considerable amount of fossil fuel and electrical energy is consumed. on the other hand for generating one tone of portland cement, nearly one ton of carbon dioxide is released. it shows that 7 percent of the total released carbon dioxide in the world relates to the cement industry. considering ecological issu...

the effect of using visual aids on the development of speech act of disagreement among iranian intermediate efl learners

abstract the present study tried to investigate the effect of visual aids (films) on the development of the speech act of disagreement among iranian efl intermediate learners. to this end, the researcher selected 40 homogeneous intermediate learners based on their scores on oxford placement test. .the subjects then divided into control group and experimental group. both classes were tested by ...

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 8  issue 2.5

pages  139- 139

publication date 2014-07-01

By following a journal you will be notified via email when a new issue of this journal is published.

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023