Molecular investigation of the prevalence and detection of enterohemorrhagic Escherichia coli isolated from traditional cheese in Iran
Authors
Abstract:
Introduction: Shiga toxin-producing Escherichia coli (STEC) strains are one of the main causes of food poisoning and diseases such as hemorrhagic colitis and hemolytic uremic syndrome. Food contamination mainly occurs through food of animal origin such as meat and dairy products. The aim of this study was to investigate prevalence and distribution of virulence genes in Escherichia coli isolates from traditional cheese from Semnan city, using multiplex PCR method. Materials and Methods: In this study, a total of 100 traditional cheese samples were screened for E. coli strains through conventional microbiological tests. Then, screening for virulence genes (Stx1, Stx2, eaeA and hlyA genes) was carried out by using PCR method. Results: Fifty-nine Escherichia coli strains were identified among 100 cheese samples. Of these, 55 isolates exhibited for one or more virulence genes (93.2%) which, 44 isolates carrying more than of one gene and 11 isolates were positive for the carrier of one gene. Stx2 showed the highest frequency of virulence gene in E. coli isolates. Conclusion: Therefore, due to the high prevalence of virulence genes in pathogenic E. coli isolates in traditional cheese, it is necessary to use molecular methods to control the microbial quality of these products.
similar resources
Detection of Methicillin-Resistance Gene in Staphylococcus aureus Isolated from Traditional White Cheese in Iran
Background & Aims of the Study: Methicillin-resistant staphylococcus aureus (MRSA) is considered as a major pathogen in public health concern. The objectives of this study were to firstly determine antibiotic sensitivity among Staphylococcus aureus isolated from traditional Iranian white cheese during 2015 from Hamedan province of Iran; and secondly to estimate the presence of ...
full textPrevalence, Molecular Characterization and Serology of Shiga toxin-Producing Escherichia coli Isolated from Buffaloes in West Azerbaijan, Iran
This present study is the first to report the presence of Shiga toxin-producing Escherichia coli (STEC) in buffaloes in Iran. A total of 360 fecal samples were collected from buffaloes from different regions in the west Azerbaijan province of Iran and cultured for the isolation of E. coli using routine biochemical tests. From the fecal samples, 340 E. coli were isolated and, of these, 26 STEC i...
full textthe role of russia in transmission of energy from central asia and caucuses to european union
پس ازفروپاشی شوروی،رشد منابع نفت و گاز، آسیای میانه و قفقاز را در یک بازی ژئوپلتیکی انرژی قرار داده است. با در نظر گرفتن این منابع هیدروکربنی، این منطقه به یک میدانجنگ و رقابت تجاری برای بازی های ژئوپلتیکی قدرت های بزرگ جهانی تبدیل شده است. روسیه منطقه را به عنوان حیات خلوت خود تلقی نموده و علاقمند به حفظ حضورش می باشد تا همانند گذشته گاز طبیعی را به وسیله خط لوله مرکزی دریافت و به عنوان یک واس...
15 صفحه اولthe survey of the virtual higher education in iran and the ways of its development and improvement
این پژوهش با هدف "بررسی وضعیت موجود آموزش عالی مجازی در ایران و راههای توسعه و ارتقای آن " و با روش توصیفی-تحلیلی و پیمایشی صورت پذیرفته است. بررسی اسنادو مدارک موجود در زمینه آموزش مجازی نشان داد تعداد دانشجویان و مقاطع تحصیلی و رشته محل های دوره های الکترونیکی چندان مطلوب نبوده و از نظر کیفی نیز وضعیت شاخص خدمات آموزشی اساتید و وضعیت شبکه اینترنت در محیط آموزش مجازی نامطلوب است.
Antibiotic susceptibility assessment of Escherichia coli isolated from traditional cheeses in Marand, Iran
Background and Aim: Dairy products, especially cheese, are a suitable environment for the growth of pathogenic bacteria that cause disease after being transmitted to humans. Escherichia coli is one of the microbial agents in traditional cheese that causes human disease. The aim of this study was to evaluate the contamination rate of traditional cheeses with E.coli and to determine their antibio...
full textAntibiotic susceptibility assessment of Escherichia coli isolated from traditional cheeses in Marand, Iran
Background and Aim: Dairy products, especially cheese, are a suitable environment for the growth of pathogenic bacteria that cause disease after being transmitted to humans. Escherichia coli is one of the microbial agents in traditional cheese that causes human disease. The aim of this study was to evaluate the contamination rate of traditional cheeses with E.coli and to determine their antibio...
full textMy Resources
Journal title
volume 23 issue 1
pages 174- 179
publication date 2021-01
By following a journal you will be notified via email when a new issue of this journal is published.
No Keywords
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023