Molecular detection of hepatitis delta virus in blood donors with RT-PCR
Authors
Abstract:
Abstract Background and Objective: Hepatitis delta virus is an imperfect virus with RNA and its activity depends on the presence of hepatitis B virus. This virus can lead to acute and chronic diseases in the liver. This study aimed to detect the hepatitis delta virus in blood donors with positive Hepatitis B Surface Antigens (HBsAg). Material and Methods: In this Study, 350 serum samples were obtained from the people infected with hepatitis B blood in Transfusion organization of Shahrekord city, Iran. After extracting RNA by RNA Plus kit and making cDNA, the samples were evaluated by using RT PCR. Results: Of 350, two samples (0.57%) were infected by HDV. Conclusion: Low prevalence of HDV infection shows that Hepatitis B is being controlled in Shahrekord. Keywords: Hepatitis Delta Virus, Blood Donors, Hepatitis B Surface Antigens
similar resources
Detection and Genotyping of Hepatitis D Virus from HBsAg Positive Patients in Iran Using RT-PCR
Hepatitis Delta virus (HDV) is a degenerate RNA virus or virusoid and a satellite of Hepatitis B virus (HBV). Three distinct genotypes are described for HDV; genotype I is distributed worldwide but other genotypes appear to be more restricted geographically. In the present study, an RT-nested PCR method was set up to detect delta infection from serum samples. Moreover, the target amplified sequ...
full textHepatitis C virus infection and genotypes in blood donors
Abstract: Genotyping of the hepatitis C virus (HCV) is important for designing therapeutic strategies and regional specific diagnostic assays. The aim of this study was to identify the HCV genotypes in HCV infected blood donors. This is the first report on HCV genotypes in blood donors in Iran. In this cross-sectional study, 103 blood donors with hepatitis C were investigated for HCV genotype...
full textHepatitis E Virus and Serum Level Aminotransferases in Blood Donors
Background: Hepatitis E virus (HEV) infection is a self-limiting viral infection that can lead to severe complications and death. In different regions the epidemiology of this infection varies. In this study we evaluated the seroepidemiology of hepatitis E infection in Jahrom, a city in southern Iran. Methods: This was a cross-sectional descriptive study of serum samples from 477 subjects, i...
full textDecline of Hepatitis B Virus in Iranian Blood Donors in the Last Decade (2001-2010)
Background and Aims: This study was the trends of Hepatitis B infection among Iranian blood donors which was analyzed in a period of 10 years. Materials and Methods: In a period of 10 years, from 2001 through 2010, a total of 16,264,830 donations from 30 regional and 35 local blood services were screened. All blood samples were tested for Hepatitis B surface antigen (HBs Ag) by commercial avail...
full textseroprevalence and molecular detection of hepatitis delta virus (hdv) among hemodialysis patients and blood donors in a cross-sectional study in khartoum state, sudan
background the hepatitis delta virus (hdv) was first discovered by rizzetto in 1977 in a patient with chronic hepatitis b virus (hbv) infection. in 1980, it was shown that hdv was an infectious agent responsible for exacerbation of liver disease in patients with hepatitis. objectives the aim of this study was to determine the seroreactivity of, and to molecularly detect, hdv among hemodialysis ...
full textRisk factors of hepatitis B virus infection among blood donors in Duhok city, Kurdistan Region, Iraq
Background: Hepatitis B virus (HBV) infection is a public health problem. The lack of information about the seroprevalence and risk factors is an obstacle for preventive public health plans to reduce the burden of viral hepatitis. Therefore, this study was conducted in Iraq, where no studies had been performed to determine the prevalence and risk factors of HBV infection. Methods: Blood sample...
full textMy Resources
Journal title
volume 9 issue 2
pages 26- 31
publication date 2015-07
By following a journal you will be notified via email when a new issue of this journal is published.
No Keywords
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023