Isolation, Identification and Antibiotic Resistance Profile of Listeria Monocytogenes Strains in Red Meats

Authors

  • Amini, J, Islamic Azad University, Kerman Branch, Iran.
  • Ebrahimi, E, Islamic Azad University, Jahrom Branch, Iran
  • Kargar, M, Jahrom Branch, Islamic Azad University, Jahrom, Iran.
  • Kheirkhah, B, Islamic Azad University, Kerman Branch, Iran
  • Najafi, A, The Persian Gulf Marine Biotechnology Research Center, Bushehr University of Medical Sciences, Bushehr, Iran
Abstract:

Abstract Background and Objective: Listeria monocytogenes is a bacterium transferred by foods and is the agent of many sporadic and epidemic diseases in humans. This study aimed to investigate the prevalence of L. monocytogenes and to determinine their antibiotic resistance profile in red meats. Material and Methods: this cross-sectional study was performed on 400 red meat samples obtained from industrial slaughterhouses placed in Kerman, Iran. First, the samples were enriched with Simultaneous Enrichment Broth (SEB), and then plated onto Palcam agar and Tryptic Soy Broth Yeast Extract Broth (TSAYE). After identification of the isolates based on biochemical tests and PCR, the isolates were checked for their antibiotic resistance profile using disk Diffusion Results: of 400 samples, 12 samples (3%) were contaminated with different species of Listeria. Using PCR, hly gene was recognized in eight samples (2%) of L. monocytogenes. Furthermore, there was a significant difference in isolation rate of lamb samples compared to cow ones. While all of the isolates were resistant to clindamycin, amikacin and chloramphenicol, they were sensitive to penicillin. Conclusion: in spite of low rate of infection in red meat samples in Kerman city, due to high risk of Listeria contamination in red meats, we recommend applying a routine screening to identify this bacterium in our county. Keywords: Listeria Monocytogenes, Hly Gene, Red Meat, Antibiotic, Kerman

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

Isolation, Identification, and Profile of Antibiotic Resistance of Bacteria in Patients with Cancer

Background: Blood stream infection is one of the main causes of morbidity and mortality in patients with cancer. The purpose of this study was to evaluate the prevalence of aerobic and anaerobic-bacteremia in hospital inpatients with cancer and to determine the antibiotic resistance profile in isolated organisms in Kerman, southeast Iran. Methods: Total of 240 blood cultures from 136 patients w...

full text

Prevalence and Antibiotic Resistance of Listeria Monocytogenes in Chicken Meat Retailers in Yazd, Iran

Introduction: Listeria monocytogenes is a pathogen bacteria transmitted by food stuffs. Due to the lack of information about contamination of retail chicken meat to Listeria monocytogenes in Iran, this study aimed to determine the prevalence and antibiotic resistance of this bacterium in retail chicken meat in Yazd, Iran. Materials and Methods: This cross-sectional descriptive study was conduc...

full text

Importance of Listeria monocytogenes in food safety: a review of its prevalence, detection, and antibiotic resistance

Listeria monocytogenes, as a foodborne pathogenic bacterium, is considered as major causative agent responsible for serious diseases in both humans and animals. Milk and dairy products are among the main sources of energy supply in the human, therefore contamination of these products with Listeria spp., especially L. monocytogenes, could lead to life threatening infec...

full text

Occurrence and Antibiotic Resistance of Listeria monocytogenes in Retail Minced Beef Distributed in Ahvaz, South-West of Iran

Background: Listeria monocytogenes is one of the most important food-borne bacteria causing septicemia, meningitis and encephalitis in humans. The objective of this study was to evaluate the occurrence and antibiotic resistance of the bacterium in retail minced beef in Ahvaz, South-West of Iran. Methods: In this survey, 150 samples of minced beef were randomly obtained from retail butcheries i...

full text

Isolation, Molecular Identification, and Antibiotic Resistance Profile of Salmonella Typhimurium Isolated from Calves Fecal Samples of Dairy Farms in Hamedan

Introduction: Calf diarrhea is one of the significant problems in dairy farms associated with treatment costs and reduced livestock production. Salmonellae are among the most common and the major causative agents of diarrhea in calves and humans. The present study was carried out to isolate and identify Salmonella in fecal samples of calves in industrial dairy farms of Hamedan and to determine ...

full text

Isolation of Listeria monocytogenes from Meat and Dairy Products

Introduction: This study was intended to determine the presence and distribution of Listeria monocytogenes in various meat and dairy products from Qazvin Province by culture followed by biochemical and morphological assays. The identity of the isolates was further obtained by amplification of prfA gene in bacteria isolates. This gene is a transcriptional activator of virulence gene ex...

full text

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 8  issue 4

pages  27- 33

publication date 2015-01

By following a journal you will be notified via email when a new issue of this journal is published.

Keywords

No Keywords

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023