Isolation and identification of new beneficial bacterial strains from rhizosphere of Citrus sinensis orchards

Authors

  • Ali Riahi Madvar Department of Biotechnology, Institute of Science and High technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran
  • Mahnaz Ramezani Department of Biology, Science and Research branch, Islamic Azad University, Kerman, Iran
  • Moj Khaleghi Department of Biology, Faculty of Sciences, Shahid Bahonar University, Kerman, Iran
  • Roohullah Hemmati Department of Biochemistry, faculty of biological sciences,Tarbiat Modares University, Tehran, Iran
Abstract:

The rhizosphere is the area around the root of a plant occupied by a unique population of usefulbacteria known as plant growth promoting rhizobacteria (PGPR). In this study, the isolation andidentification of rhizobacteria from orange (Citrus sinensis) orchards using 16S rRNA gene, as well asbiological and biochemical assays is reported. Analysis of 16S rRNA gene was confirmed bybiological and biochemical assays and showed that the isolated bacteria belong to the genera Bacilli,Enterobacter and Pseudomonas. Accordingly, Enterobacterkobei MR-R2, Pseudomonas putida MRR5,Bacillus cereus MR-R, Bacillus thuringiensis MR-R1 and Bacillus mycoides MR-R4 wereidentified and registered in GenBank. The results of phosphate solubilization testing revealed that themaximum rate of phosphate solubilization was observed for B. thuringiensis, and E. kobei; however,B. cereus, B. mycoides, and P. putida indicated the least activity. It can be concluded that the isolatedstrains (especially B. thuringiensis) are newly identified phosphate solubilizing bacterial strains,among which B. thuringiensis can be used for growth promotion of orange trees.

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isolation and identification of new beneficial bacterial strains from rhizosphere of citrus sinensis orchards

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Journal title

volume 5  issue 1

pages  75- 84

publication date 2015-06-01

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