Investigation on some main glycosides content of Stevia rebaudian B. under different concentration of commercial and synthesized silver nanoparticles

Stevia rebaudiana B. extracts as a low-calorie sweetener have gained worldwide approval. Synthesized nanoparticles by plant were used in different programs such as medical, pharmaceutical and agricultural filed. Here, we report the increase of the main glycosides of stevia plant with silver nanoparticles (AgNPs) treatment. This fact has stimulated the research on the analysis and comparison of different concentrations of commercial and synthesized AgNPs using ethanol extract on the glycosides of stevia plant by HPLC. After  AgNPssynthesis , spectrum absorption of extracts were analyzed in different ranges from 300 to 700 nm and the maximum peak was observed at 420 nm. TEM micrograph of synthesized AgNPs from Stevia rebaudiana extract showed spherical shape with the size of 25 nm. A comparison between quantities of glycoside by HPLC under both treatment of AgNP exhibited that synthesized AgNP has more effect than commercial AgNP on the stevioside and then rebaudioside. On the other hand, results showed that increasing the AgNP concentration in both treatments, will lead to glycoside content enhancement. Overall, significant changes were observed in the glycosides in plants under synthesized and commercial AgNP that could be used to create plants with higher quality of glycoside for pharmaceutical approaches.