Induction of resistance to Cadmium heavy metal stress in the produced seeds of pea after inoculation by pseudomonas bactery and boron nano chelate

In order to evaluate the effect of Inoculation by pseudomonas plant growth promotinh rhizobacteria and  foliar application of boron-nanochelate of pea( Pisum sativum L.) mother plants on germination traits and seedling growth of produced seeds under Cadmium stress a factorial experiment based on completely randomized design with four replications was conducted. Experimental treatments were 12 combination of foliar application  of boron-nanochelate (control, 2 and 4  mg/L), inoculation   by plant growth promoting rhizobacteria pseudomonas  strains (without inoculation, p15, p25 and p32) and three levels of cadmium stress (0, 50, 150 mg/l Cdcl2). Through this experiment traits such as seed germination and seedling growth criterias , some of biochemical parameters such as proline,  protein and soluble solid content, photosynthetic pigment content and peroxidase antioxidant enzyme were measured. Results  indicated that as increases in  cadmium  stress  germination percentage and rate seedling length and dry weight, seedling length index, photosynthetic pigments and  soluble solid and protein content of seedlings and peroxidase antioxidant enzyme activity  were decreased, while proline content of seedling were increased. Whearse     Boron nano-chelate nutrition and pseudomonas bacterial strains inoculation alleviated deterimental effects of cadmium stress via increase in seed germination and seedling growth criteria, protein  and soluble solid content, chlorophyll a and chlorophyll b  and peroxidase enzyme activity .