Increasing in the Extraction Yield of Environmentally Friendly Antifouling Agent from Pseudomonas Aeruginosa MUT3 by Response Surface Methodology (RSM)

In the present study, the solvent-solvent extraction of phenazine 1-carboxylic acid (PCA) as an environmentally friendly antifouling agent from pseudomonas aeruginosa MUT3 culture was investigated. Accordingly, after screening the extraction ability of various solvents, the combined effects of operating parameters such as solvent type (ethyl acetate, dichloromethane, and n-hexane), solvent percent and mixing time on the PCA extraction process were analyzed using response surface methodology (RSM). As a consequence, ethyl acetate showed higher extraction yield (68%) and the optimum condition for PCA extraction were identified as 150% of solvent and 120 min mixing time. Meanwhile, the extraction yields for dichloromethane and n-hexane were measured by HPLC assay around 48.75 and 25.2%, respectively. The accuracy of the obtained model was proved by 99.90% R2 and 99.84% Adj R2. In addition, the disk diffusion test showed 9.2, 8 and 7.3 mm inhibition zone for ethyl acetate, dichloromethane and n-hexane, respectively. Consequently, the present study provided a great insight into the solvent-solvent extraction of antibiotics from the fermentation broth.