In Vitro Evaluation of Protective Effect of Rutin on Acrylamide-Induced Cellular Senescence in NIH3T3 Cells

Background: Aging is one of the important factors in the development of age-related diseases. Acrylamide can be produced during carbohydrate-rich foods prepared at high temperatures. Recently, studies showed that acrylamide can induce cellular senescence. On the other hand, Rutin as a natural flavonoid, has a potent antioxidant activity. Objective: This study aims to evaluate the ptotective effect of Rutin on oxidative-induced cellular senescence induced by acrylamide. Methods NIH3T3 mouse embryonic fibroblast cells were used in this study, which treated by different concentrations of acrylamide and Hydrogen Peroxide (H2O2). Oxidative stress was assessed by measuring the malondialdehyde and glutathion concentrations. To evaluate the senescence process, β-galactosidase activity was measured by enzyme staining and β-galactosidase activity assay kit. The MTT assay was used to evaluate the cell viability.  Results: Exposure of cells to acrylamide and H2O2 significantly reduced the cell viability, and Rutin significantly improved cell viability in cells treated by acrylamide (P<0.05). Rutin also increased glutathion level in cells treated by acrylamide and H2O2 (P<0.05). The rate of lipid peroxidation was significantly lower in the group treated by rutin and H2O2 than in the H2O2 group (P<0.05).The β -galactosidase activity was increased in the H2O2 and acrylamide groups. The use of rutin with acrylamide significantly reduced the activity of β-galactosidase enzyme compared to the acrylamide group (P<0.05).  Conclusion: Oxidative stress may be an important mechanism in acrylamide-induced  senescence in NIH3T3 cells. On the other hand, Rutin, as a potent antioxidant, can reduce cellular senescence by inhibition of oxidative damage.