Evaluation of the effects of concentration, duration and temperature of incubation with Beta-Propiolactone on inactivation of cell-derived rabies virus

Authors

  • Eftekhari, Z Research and Production Complex, Pasteur Institute of Iran. Alborz, Iran.
  • Hosseini , SM Research and Production Complex, Pasteur Institute of Iran. Alborz, Iran.
  • Jodairi Eslami , S Research and Production Complex, Pasteur Institute of Iran. Alborz, Iran.
  • Mehrbod, P Influenza and Other Respiratory Virus Dept, Pasteur Institute of Iran, Tehran, Iran.
  • Vaez, J Research and Production Complex, Pasteur Institute of Iran. Alborz, Iran.
Abstract:

Introduction: During the production process of vaccines against viral diseases, virus inactivation plays a crucial role.  β-Propiolactone (BPL) is a compound that is frequently used for inactivation of viruses and production of the vaccines against viral diseases such as rabies. In this study, the effects of concentration of BPL as well as the duration and temperature of incubation with BPL on the infectivity of the rabies virus was investigated. Methods: Fixed rabies (Pasteur Virus) PV strain grown on BHK21C13 cell was treated with 1:3000, 1:4000 and 1:5000 final concentrations of BPL at 4°C, 21°C and 37°C and their effects were evaluated after 24, 48, 72 h and 5 days. Each sample was titrated after various intervals and tested for the viral infectivity in BSR cells. The BSR cells were infected by triple serial dilutions of the rabies virus PV strain, and then infectivity was detected by the fluorescent technique. Results: Entire loss of infectivity of the virus treated at 4°C with 1:3000 and 1:4000 of BPL was evident at the end of the 24 and 48 h incubation periods, respectively. Moreover, the loss of infectivity of the virus treated with all tested concentrations of BPL was evident at the end of the 72 h at 21°C and 4°C. Conclusion: These findings suggest that aside from BPL concentration, temperature plays the most important role on BPL inactivation. The inactivation effect could be observed at the end of 72 h incubation period; however, the best economic condition for rabies virus inactivation was shown to be 1:4000 at 4°C for 48 h.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

the effects of planning on accuracy and complexity of iranian efl students’ written narrative task performance

this study compared the different effects of form-focused guided planning vs. meaning-focused guided planning on iranian pre-intermediate students’ task performance. the study lasted for three weeks and concentrated on eight english structures. forty five pre-intermediate iranian students were randomly assigned to three groups of guided planning focus-on-form group (gpfg), guided planning focus...

15 صفحه اول

the effects of time planning and task complexity on accuracy of narrative task performance

هدف اصلی این تحقیق بررسی تاثیر برنامه ریزی زمانی، هم چنین افزایش میزان پیچیدگی تکالیف در نظر گرفته شده بصورت همزمان، بر دقت و صحت و پیچیدگی عملکرد نوشتاری زبان آموزان می باشد. بدین منظور، 50 نفر از دانش آموزان دختر در رده ی سنی 16 الی 18 سال به عنوان شرکت کنندگان در این زمینه ی تحقیق در نظر گرفته شدند و به دو گروه آزمایشی و کنترل بصورت اتفاقی تقسیم شدند. اعضای گروه آزمایشی هر دو تکلیف ساده و پی...

the evaluation of language related engagment and task related engagment with the purpose of investigating the effect of metatalk and task typology

abstract while task-based instruction is considered as the most effective way to learn a language in the related literature, it is oversimplified on various grounds. different variables may affect how students are engaged with not only the language but also with the task itself. the present study was conducted to investigate language and task related engagement on the basis of the task typolog...

15 صفحه اول

the effects of previewing and providing background knowledge on iranian university efl learners’ comprehension of literary and academic texts

abstract poor reading comprehension can result in failure in using references, benefiting from professional gatherings and resources, keeping up with the growing body of knowledge in the virtual world of the internet, and failing to achieve in efl programs. the purpose of the present quasi-experimental study was to explore the effects of background knowledge and previewing narrative and expos...

15 صفحه اول

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 3  issue 3

pages  64- 67

publication date 2016-11

By following a journal you will be notified via email when a new issue of this journal is published.

Keywords

No Keywords

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023