Effect of Neurotrophin-3 on Differentiation of Rat Hair Follicle Stem Cells into Neural Like Cells
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Abstract:
Purpose: The aim of this study was to evaluate the effect of NT-3 on the decrease of the differentiation time of bulge stem cells of rat hair follicle from neuron like cells.Materials and Methods: The bulge region of the rat whisker was isolated from and cultured in DMEM/F12 supplemented with epidermal growth factor (EGF) in 3 groups for 7, 8 and 9 days .Then 10 ng/ml NT-3 was added to each group for 3 days. Finally, cell differentiation was assessed by immunocytochemistry using βⅢ-tubulin antibody and the result was compared with control groups.Results: According to our results hair follicle bulge stem cells expressed CD34 and Nestin in 7-9 and 10-12 first days after cultivation respectively. By using NT-3 duration differentiation process the cells showed positive reaction to βⅢ-tubulin antibody.Conclusion: The results indicate that NT-3 can affect on differentiation speed even in less than 10 days
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volume 9 issue None
pages 0- 0
publication date 2012-02
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