Effect of indomethacin on the oxidative stress of Cyprinus carpio using of biomarkers

In recent years, drugs have been identified as newly emerging and threatening the aquatic and terrestrial environments. Accordingly, in this study, the effect of indomethacin on oxidative stress in Cyprinus carpio was investigated. In order to determine the LC50, the fishes were exposed to various concentrations of indomethacin and daily losses were recorded during hours. In the next step, the fishes were exposed to various concentrations of indomethacin in five treatments including T1 (control), T2 (0.01 mg), T3 (0.02 mg), T4 (0.05 mg), T5 (0.1 mg) for 28 days. At the end of the experiment, antioxidant enzymes such as catalase (CAT), glutathione peroxidase (GPX) and malondialdehyde (MDA) were measured in the liver and gill tissues treated fishes.the lethal toxicity (LC50) of indomethacin was obtained for carp (328.49 mg/l). GPX enzyme activity in gill and liver tissue in all treatments increased significantly in compared to the control group (p<0.05). MDA levels in liver tissue in T4 and T5 groups and gill tissue in T3 and T4 groups showed a significant increase in compared to the control group (p<0.05). Also, CAT activity in liver and gill tissue in T4 and T5 groups was increased significantly in compared to the control group (p<0.05).The results of this study demonstrated that presence indomethacin in an aqueous environment can influence the antioxidant status and health of the fishes.