Effect of Different Concentration and Application Methods of Polyamines (Putrescine, Spermine, Spermidine) on Some Morphological, Physiological, and Enzymatic Characteristics and Vase Life of Rosa hybrida cv. ‘Dolce Vita’ Cut Flower

In order to study on different methods of application of polyamines (putrescine, spermine, spermidine) on some morphological, physiological, enzymatic and vase life of Rosa hybrida cv. ‘Dolce Vita’, experiment was conducted as a factorial experiment in a completely randomized design with 32 treatments, 3 replicates and each replication containing 5 vases, a total of 480 vases were executed. First factor was, putrescine, spermine, spermidine (50, 100 mg/l) and second factor was 4 methods of uses (pre-harvest spray, postharvest spray, 24 hrs pulse and continuous). We are 2 blanks in this experiment: distilled water and 3 % sucrose + 3 mg l-1 nanosilver. Morphological, physiological and enzymatic traits such as relative fresh weight, cell membrane stability index, anthocyanin of petals, total chlorophyll of leaves, protein, phenylalanine ammonia- lyase and superoxide dismutase enzymes activity and vase life were measured. The results showed that Spm100 mg/l in pre-harvest spray method had the highest rates in protein, activity of phenylalanine ammonia- lyase and superoxide dismutase enzymes. Therefore, according to the results of this study, the method of pre-harvest spraying of polyamines can be recommended as the best way to improve morphological, physiological, enzymatic and vase life of Rosa hybrida cv. ‘Dolce Vita’.