Differential WNT Gene Expression in Various Subtypes of Acute Lymphoblastic Leukemia

Authors

  • Ali Memarian Department of Immunology, School of Public Health
  • Fazel Shokri Department of Immunology, School of Public Health | Monoclonal Antibody Research Center, Avicenna Research Institute, Tehran, Iran
  • Hossein Asgarian-Omran Department of Immunology, School of Public Health
  • Mahdi Shabani Monoclonal Antibody Research Center, Avicenna Research Institute, Tehran, Iran
  • Mina Tabrizi Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences
  • Parvaneh Vosough Clinic of Hematology and Oncology, Ali-Asghar Hospital, Faculty of Medicine
Abstract:

Background: Dysregulation of WNT signaling has been reported in many malignancies. Objective: This study was conducted to investigate the expression pattern of 14 members of the WNT gene family in different immunophenotypic subtypes of ALL. Methods: Semi-quantitative RT-PCR was performed on samples from 71 ALL patients and 36 age-matched healthy individuals. The ALL patients were categorized into BALL (76%), T-ALL (22.6%) and mixed lineage (1.4%) and the B-ALL cases were further classified into pro-B, pre-BI, pre-BII and immature/mature-B based on immunophenotypic results. Results: Among the WNT genes, WNT-7B (p=0.026), WNT-9A (p=0.020) and WNT-16B (p=0.023) were significantly over-expressed, whereas WNT- 2B (p=0.033), WNT-5A (p=0.016), WNT-7A (p

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

differential wnt gene expression in various subtypes of acute lymphoblastic leukemia

background: dysregulation of wnt signaling has been reported in many malignancies. objective: this study was conducted to investigate the expression pattern of 14 members of the wnt gene family in different immunophenotypic subtypes of all. methods: semi-quantitative rt-pcr was performed on samples from 71 all patients and 36 age-matched healthy individuals. the all patients were categorized in...

full text

Expression of ROR1 Gene in Patients with Acute Lymphoblastic Leukemia

Background: Acute lymphoblastic leukemia (ALL) results from genetic alterations in a single lymphoid progenitor cell. Expression of ROR1 is reported to be increased in ALL and mantle cell lymphoma. In this study the expression of ROR1 was assessed in newly diagnosed patients with ALL.  Methods: This study was carried out on 40 patients with newly diagnosed ALL and healthy individuals as contro...

full text

Significance of Cross Lineage Antigen Expression in Acute Lymphoblastic Leukemia

Background: Aberrant expression of cross-lineage antigens gives valuable insight into the diagnosis and prognosis of acute leukemia. In countries like India, cytogenetic tests are widely accessible. Exploring the prognostic value of an accessible test is of great importance. Therefore, establishing a population-specific immunophenotype database will enable to design an antibody panel equipped t...

full text

Expression Analysis of Foxo3a Gene in Pediatric Acute Lymphoblastic Leukemia in Southern Iranian Population

Background: Acute lymphoblastic leukemia (ALL), the most common childhood cancer with a peak incidence in children from 2-5 years old, might be associated with poor prognosis and resistance to therapy in specific cytogenetic backgrounds. FoxO3a, a member of the forkhead class ‘O’ (FoxO) transcription factors, is a main downstream target of PI3K/AKT pathway which regulates different ...

full text

PROGNOSTIC VALUE OF MYELOID ANTIGEN EXPRESSION IN ACUTE LYMPHOBLASTIC LEUKEMIA

Expression of cell surface molecules associated with lymphoid and myeloid lineage differentiation on the blasts of 53 patients with acute lymphoblastic leukemia (ALL) was investigated. 1.9% of cases were only HLA"7DR+ 7.6% were HLA-DR+, CDI9+ 22.6% were HLA-DR+, CDI9+, CDI0+ 30.2% were HLADR+, CDI9+, CDlO±, CD20+ and 37.7% were HLA-DR±, CD7+, CD5±. Aberrant expression of one or more myeloi...

full text

Study of Gene Expression Signatures for the Diagnosis of Pediatric Acute Lymphoblastic Leukemia (ALL) Through Gene Expression Array Analyses

Background: Acute lymphoblastic leukemia (ALL) as the most common malignancy in children is associated with high mortality and significant relapse. Currently, the non-invasive diagnosis of pediatric ALL is a main challenge in the early detection of patients. In the present study, a systems biology approach was used through network-based analysis to identify the key candidate genes related to AL...

full text

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 9  issue 1

pages  61- 71

publication date 2012-03-01

By following a journal you will be notified via email when a new issue of this journal is published.

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023