Detection of TEM Gene in Clinical Isolates of Pseudomonas aeruginosa and Escherichia coli in Isfahan province (from 2018)

Authors

  • Amir Eshaghiyan Department of Genetics, Arsanjan Branch, Islamic Azad University, Arsanjan, Shiraz, Iran
  • Behnaz Nateghi Department of Biochemistry, Faculty of Science, Nourdanesh Institutions of Higher Education, Meimeh, Isfahan, Iran
  • Elahe Shams Young Researchers and Elite Club, Falavarjan Branch, Islamic Azad University, Isfahan, Iran
  • Parisa Behshood Young Researchers and Elite Club, Falavarjan Branch, Islamic Azad University, Isfahan, Iran
Abstract:

Background: The isolation of the TEM gene in Pseudomonas aeruginosa and Escherichia coli and the antibiotic resistance pattern provides useful information on the epidemiology and factors involved in these infections. Aim: The aim of this study was to evaluate antibiotic resistance and abundance of a beta-lactamase gene (TEM) in P.aeruginosa and E.coli isolated from clinical specimens by the polymerase chain reaction. Materials and methods: In this study, 120 samples of P.aeruginosa and 86 samples of E.coli isolated from urine and sputum were identified using biochemical methods. Antimicrobial resistance pattern was investigated by disc diffusion method (Kirby-bayer). Then ESBL phenotypic study was performed using a combined disk method. Finally, the TEM gene was examined in the strains by polymerase chain reaction. Results: In this study, the highest resistance to P.aeruginosa samples was detected with tetracycline and amoxicillin antibiotics with 97.5% and 95% frequency. The highest sensitivity to the aztreonam and amikacin antibiotics was 73.33% and 61.66%, respectively. Also, 56.66% of samples had TEM gene. In E.coli samples, the highest resistance to antibiotics such as Co-trimoxazole and Amoxicillin was 59.34% and 55.04% and observed the highest sensitivity to Imipenem antibiotics was 69.66% and Chloramphenicol with 61.92% and 72.09% of the samples had TEM gene. Conclusions: These findings indicate an increase in bacterial resistance to antibiotics. So, the need to decide on the rational use of drugs and the importance of using new diagnostic methods and molecular methods to identify ESBL producing strains in microbiological laboratories.  

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publication date 2019-02

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