Detection of avian leukosis virus subgroup J in albumen of commercial and native fowl eggs using RT-PCR in Fars province of Iran

Authors

  • A. Mohammadi Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
  • H. Dadras Department of Avian Medicine, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
  • M. Rajabzadeh Graduated from School of Veterinary Medicine, Shiraz University, Shiraz, Iran
Abstract:

The subgroup J of ALV (ALV-J) has emerged as an important pathogen of meat-type chickens since1989. This virus is responsible for economic losses due to both mortality and depressed performance inchickens. So, the objective of this study is the detection of ALV-J in the albumen of commercial and nativefowl eggs using RT-PCR. Three hundred and seventy egg albumens were randomly selected from differentfarms of Fars province, Iran. These eggs were obtained from the flocks of two research centers on native fowl production (70 eggs), a broiler grandparent farm (60 eggs), three broiler breeder farms (180 eggs), and a commercial layer flock (60 eggs). RT-PCR was undertaken on isolated RNA from egg samples using a pair of ALV-J specific primers H5/H7 that produced a 545 basepair fragment. RT-PCR analyses detected ALV-J in 15 of 180 (8.33%) samples from three broiler breeders farms, 17 of 70 (24.28%) samples from flocks of two research centers of native fowls production, and none of the samples of commercial layer and broiler grandparent farms. Direct sequencing using primers specific for subgroup ALV-J verified the viral subgroup in the RT-PCR amplification products. This is the first report of the ALV-J in egg albumen in Iran which indicates the necessity to apply eradication programs for ALV-J in the poultry industry and native fowls in Iran.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

detection of avian leukosis virus subgroup j in albumen of commercial and native fowl eggs using rt-pcr in fars province of iran

the subgroup j of alv (alv-j) has emerged as an important pathogen of meat-type chickens since1989. this virus is responsible for economic losses due to both mortality and depressed performance inchickens. so, the objective of this study is the detection of alv-j in the albumen of commercial and nativefowl eggs using rt-pcr. three hundred and seventy egg albumens were randomly selected from dif...

full text

Detection of avian leukosis virus (ALV) in albumen of Shiraz commercial and local layer flocks using ELISA and RT-PCR

Avian leukosis viruses (ALVS) cause different types of tumours in poultry and can affect the health and egg production of the birds. To investigate the presence of the virus in chicken layer flocks in Shiraz, 222 egg albumen from local layer breeder (25 eggs), local layer grand parent (30 eggs), broiler breeder (60 eggs), commercial layer (46 eggs) and broiler grand parent (61 eggs) were tested...

full text

detection of avian leukosis virus (alv) in albumen of shiraz commercial and local layer flocks using elisa and rt-pcr

avian leukosis viruses (alvs) cause different types of tumours in poultry and can affect the health and egg production of the birds. to investigate the presence of the virus in chicken layer flocks in shiraz, 222 egg albumen from local layer breeder (25 eggs), local layer grand parent (30 eggs), broiler breeder (60 eggs), commercial layer (46 eggs) and broiler grand parent (61 eggs) were tested...

full text

Detection of Avian Encephalomyelitis Virus in Broiler Chickens in Iran Using RT-PCR and Histopathological Methods

Avian encephalomyelitis is caused by a Tremovirus and primarily affects chickens. The virus can infect young chicks and cause nervous symptoms. Vaccines are used to control the disease in breeders. Recently, the occurrence of the disease is causing concern in the poultry industry. In the present study, we report a case of avian encephalomyelitis in one broiler farm in Kashan (center of Iran). T...

full text

Serologic profile of avian leukosis virus subgroup-J, Mycoplasma gallisepticum and Mycoplasma synoviae in broiler grandparent flocks of Iran.

  Avian Leukosis Virus Subgroup-J (ALV-J). Mycoplasma gallisepticum (MG) and MycopJasma synviae (MS) are important pathogens in chickens that cause severe economical losses in poultry industry throughout the world. Seven broiler grandparent flocks of Iran (six broiler strains) were sampled randomly at the ages of 8-63 wk (100 samples from each flock) for antibody detection to ALV-J, MG, and MS ...

full text

role of cultural iran in promotion of multilaieralism in central asia and caucasus

cultural iran is a scope that is more extended than the political territories of iran as a political unit. this concept means that cultural geography(mehdi moghanlo-1383-1) of iran is greater than its political geography which, according to history, has a long history extending west-east from kandahar to the euphrates and north-south from the persian gulf to the caucasus including transoxiana a...

15 صفحه اول

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 12  issue 1

pages  1- 7

publication date 2011-03-20

By following a journal you will be notified via email when a new issue of this journal is published.

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023