Comparison of Interferon-Gamma (IFNG) +874 T/A Single Nucleotide Polymorphism in Hepatitis C Virus Infected Patients and Non-Infected Controls in Mashhad, Iran

Authors

  • Abbas Esmaeelzade Dept. of Internal Medicine, Qaem Hospital, Mashhad University of Medical Sciences, Mashhad, Iran
  • Alireza Pasdar Dept. of Modern Sciences and Technologies, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran | Division of Applied Medicine, Medical School, University of Aberdeen, Foresterhill, Aberdeen, AB25 2ZD, UK
  • Fariba Rezai Talab Dept. of Internal Medicine, Imam Reza Hospital, Mashhad University of Medical Sciences, Mashhad, Iran
  • Fatemeh Nategh Applied Biotechnology Research Center, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran
  • Hamed Hatami Dept. of Immunology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
  • Hamid Reza Sima Dept. of Internal Medicine, Imam Reza Hospital, Mashhad University of Medical Sciences, Mashhad, Iran
  • Hasan Vosughinia Dept. of Internal Medicine, Imam Reza Hospital, Mashhad University of Medical Sciences, Mashhad, Iran
  • Homan Mosanan Mozafari Dept. of Internal Medicine, Qaem Hospital, Mashhad University of Medical Sciences, Mashhad, Iran
  • Hosein Nomani Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
  • Mitra Ahadi Dept. of Internal Medicine, Qaem Hospital, Mashhad University of Medical Sciences, Mashhad, Iran
  • Mohammad Reza Sarvghad Dept. of Infectious Diseases, Imam Reza Hospital, Mashhad University of Medical Sciences, Mashhad, Iran
  • Mohammad Taghi Shakeri Dept. of Biostatistics, Public Health School, Mashhad University of Medical Sciences, Mashhad, Iran
  • Samaneh Sepahi Targeted Drug Delivery Research Centre, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
  • Sina Gerayli Dept. of Biology, University of Western Ontario, London, Ontario N6A5BF, Canada
  • Sina Rostami Dept. of Laboratory Medicine, Children's and Women's Health, NTNU - Norwegian University of Science and Technology, NO-7491 Trondheim, Norway
  • Zahra Meshkat Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
Abstract:

Background and Objectives: Interferon-gamma is an important cytokine, which facilitates immunity against intracellular pathogens. Several factors, including genetic variations of cytokine-producing genes have been shown to influence the progression and severity of Hepatitis C virus (HCV) infection. Methods: Between January and December 2012, 87 HCV-infected individuals and 89 individuals without HCV infection were recruited for the study of Single Nucleotide Polymorphism (SNP) at Interferon Gamma (IFNG) +874 T/A. After extraction of genomic DNA from Peripheral Blood Mononuclear Cells (PBMCs) in blood sample of the individuals, Amplification Refractory Mutation System (ARMS) polymerase chain reaction was performed to evaluate the SNP at this position. Results: The frequency of genotype TA was 62.1% in the HCV-infected group, while it was 47.2% for the control group (p=0.033). However, after adjusting for confounders (including alcohol consumption, drug addiction, transfusion, and tattoos), the genotypes at this position did not show any statistically significant association with HCV infection (adjusted P values were above 0.05). The frequency of allele A was slightly higher in patients than the controls (55.2% versus 48.3%).Carriers of A allele were more frequent in patients with HCV infection compared to the control group (55.17% in patients versus 48.31% in the control group; P=0.02). However, after adjustment for confounders, the results were no longer statistically significant (P=0.2). Conclusion: A carrier status for certain alleles and genotypes at Interferon Gamma (IFNG) +874 T/A may lead to higher susceptibility to HCV infection in a certain population.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

Hepatitis E Virus Seroprevalence and Viremia in Hemodialysis and HIV Infected Patients in Iran

Background and Aims: Hepatitis E virus (HEV) infection is usually a self-limited viral disease that causes acute hepatitis and may progress to chronic hepatitis in immunosuppressed individuals. It seems that hemodialysis patients and HIV infected people are more exposed to HEV infection. The aim of this study was to evaluate the extent of HEV infection in hemodialysis and HIV infected patients ...

full text

Comparison of Hepatitis C Genotypes in Plasma and Peripheral Blood Mononuclear Cells (PMBCs) in Hepatitis C-Infected Patients

Abstract Background and Aims: The Hepatitis C Virus (HCV) is essentially hepatotropic but virus compartment has also been found in other important extra hepatic sites. Detection of HCV RNA in extra hepatic reservoirs such as peripheral blood mononuclear cells (PBMCs) is important for determining of disease progression and effective treatment. The present study aimed to determine of differe...

full text

The Absence of Hepatitis C Virus Infection Among Patients with Hepatitis B virus in Mashhad, Iran

Background and Aims: Many studies have provided evidence for the role of hepatitis B and C viruses in the development of liver cancer. Although the routine treatment is available for both conditions, no definite guideline is available to treat patients dually infected with HBV and HCV. This study was performed to determine the frequency of HBV/HCV-coinfection in Mashhad, North-East of Iran. Mat...

full text

Detection of SENV Virus in Healthy, Hepatitis B- and Hepatitis C-Infected Individuals in Yazd Province, Iran

Background: SEN virus (SENV) is the latest virus proposed as a cause of unknown hepatitis cases. Among nine detected genotypes of the virus, genotypes D and H are more frequent in hepatitis cases of unknown origin. The aim of this study was to determine the frequency of SENV-D and SENV-H genotypes in the sera of healthy individuals and hepatitis B and C patients. Methods: Totally, 200 serum sam...

full text

Prevalence of Torque Teno Virus in Healthy Individuals and Peoples Infected with Hepatitis C Virus Living in Yazd, Iran

full text

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 12  issue 3

pages  248- 256

publication date 2017-07-01

{@ msg @}

By following a journal you will be notified via email when a new issue of this journal is published.

Keywords

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023