Characterization and DFT Studies for Green Synthesis of Silver Nanoparticles by Morphine Ampules and their Anti-proliferation Activity

This study is a green approach for the synthesis of silver nanoparticles (AgNPs) using morphine ampoules (MO) as reducing and capping agents. The toxicity effects of prepared particles were evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay on breast cancer cells. The spherical AgNPs with the average size of 50nm were synthesized and studied by UV–visible spectrum, Phase analysis of prepared samples was done by X-ray diffraction analysis (XRD), environmental scanning electron microscopy (ESEM), transmission electron microscopy (TEM), Energy-dispersive X-ray spectroscopy (EDS) and dynamic light scattering (DLS). A review mechanism has been done based on Fourier-transform infrared spectroscopy (FTIR) and density functional theory (DFT) studies to explain the reducing ability of MO as a reducing agent. The nanoparticles play key roles in various biological pathways in cells. Our results revealed that synthesized silver nanoparticles using MO are a good candidate as anti-proliferation agents for the prevention and treatment of human cancer cells and can inhibit the growth of breast cancer cells at low 16.43 μg/mL.