Characterization and Assessment of Antimicrobial Activity and Potential of Heavy Metal ion Detection of Silver Nanoparticles synthesized from Actinidia deliciosa paste using double distilled water and 70% ethanol as solvent

Green synthesis of nanoparticles using plants as sources exhibiting superiority over the physical and chemical methods that are expensive and can involve the use of toxic, hazardous chemicals, which may pose biological and environmental risks. In present study silver nanoparticles were synthesized in single step by biological method using extracts of Actinidia deliciosa paste as reducing and stabilizing agent. Extracts were prepared using two different solvents i.e. double distilled water and 70% ethanol by Hot Percolation method. These extracts were augmented with AgNO3 solution and subjected to different reaction conditions i.e. pH and temperature. Synthesized silver nanoparticles of Actinidia deliciosa paste samples were preliminary characterized by using UV-VIS spectrophotometer and also on basis of change in colour in samples. Confirmatory characterization of biologically synthesized silver nanoparticles was done by Energy-Dispersive X-ray Spectroscopy (EDS) for presence of true silver metal ions samples, Transmission Electron Microscopy (TEM) so as to find out the shape and size of silver nanoparticles. The MIC test was performed to evaluate antimicrobial activity of silver nanoparticles against E.coli, Staphylococcus aureus, Pseudomonas aeruginosa. Applicative test of biosynthesized silver nanoparticles for detection of minimum concentration of Hg2+ heavy metal ions in contaminated water sample was done by observing change in optical density with respect to fixed silver nanoparticles concentration and varied Hg2+ heavy metal ions concentration by using UV-VIS spectrophotometer at wavelength of 630 nm.