Biogenesis of silver nanoparticles using selected plant leaf extract; characterization and comparative analysis of their antimicrobial activity

Objective(s): To study the antimicrobial property of green synthesised silver nano metals with M.balbisiana, A.indica and O.tenuiflorm and their enhanced antibacterial activity, assessment of antimicrobial effect. And to explore the possible mechanism of AgNPs synthesis in the active constitutions of selected temperate plant extracts Materials and methods:Biosynthesis of AgNPs using plant extract was carried out and formation of AgNPs confirmed by perceptible observation, UV spectroscopy, Scanning electron microscope (SEM) and Dynamic light scattering (DLS) were used to characterize the AgNPs.  Results:Screening of the M.balbisiana, A.indica and O.tenuiflorm extracts was carried out using standard methods to find their constitutions. The antibacterial screening was carried out by agar well diffusion method against selected microorganisms. The absorption maxima of UV visible spectrum found in the range between 300 nm to 800 nm confirmed the formation of AgNPs. SEM images revealed relatively spherical shaped of AgNPs of biosynthesized AgNPs with mean diameter about 14.51±1.5nm in O.tenuiflourum, 09.10±1.50nm M.barbisiana and 11.00±1.50 in A indica. FTIR results expounded the functional groups of plant extract responsible for the bio-reduction of silver ions and their interaction between them. Conclusion:These results showed with changes in plants constituents are may be responsible to form nanoparticles with different size and characteristics