Biochemical Methods in Production of Three-Dimensional Scaffolds from Human Skin: A Window in Aesthetic Surgery

Authors

  • Hashemi, Seyede-Sara Burn and Wound Healing Research Center, Shiraz University of Medical Sciences, Shiraz, Iran;
  • Jowkar, Somayeh Department of Biology, Center of Taft, Payame Noor University, Taft, Iran
  • Keshavarzi, Abdolkhalegh Burn and Wound Healing Research Center, Shiraz University of Medical Sciences, Shiraz, Iran; Stem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran; Rohan Gene Cell Tech, Shiraz, Iran
  • Mahmoodi, Mahdokht Burn and Wound Healing Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
  • Mehrabani, Davood Burn and Wound Healing Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
  • Rafati, Ali Reza Burn and Wound Healing Research Center, Shiraz University of Medical Sciences, Shiraz, Iran; Division of Pharmacology and Pharmaceutical Chemistry, Sarvestan Branch, Islamic Azad University, Sarvestan, Iran
  • Zarei, Masoumeh Department of Obstetrics and Gynecology, Shiraz University of Medical Sciences, Shiraz, Iran
Abstract:

BACKGROUND Use of matrix-derived biologic scaffolds has become a treatment of choice in several clinical issues. This study assessed biochemical methods in production of three-dimensional scaffolds from human skin. METHODS Human skin was prepared from circumcisions, washed in phosphate buffer saline (PBS) and kept at -20ºC until use. The skin samples underwent various methods. In group A, NaCl, Triton X100 and EDTA solution were used for removal of epidermis and was subdivided to three subgroups. The solution for removal of epidermis was similar for all subgroups, but decellularization was different. Group B was subdivided into 6 subgroups, NaCl in different concentrations was used for removal of epidermis and decellularization happened using SDS in various concentrations and different time intervals. Group C was subdivided to 3 subgroups, trypsin was used for removal of epidermis and decellularization was conducted applying NaOH or SDS. Washing was performed using only PBS. In group D, decellularization was done applying SDS. Histomorphometric study was conducted to compare the groups. RESULTS No fibroblast was present in A2, B2, B4, and C3 subgroups after decellularization. Histological photographs from subgroups A1 to A3 revealed several cells and collagen fibers. Dense collagen fibers in pink color were noted in all subgroups; but, epidermis was absent. CONCLUSION It was shown that 1M NaCl was the best solution for removal of epidermis, 0.5% SDS for 2 h was the most effective solution for decellularization and PBS was the best solution for washing, while the solutions are easily available and cost-effective.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

Role of Cultured Skin Fibroblasts in Aesthetic and Plastic Surgery

The skin is the largest tissue of man with several functions. Cosmetics/cosmeceuticals agents always are needed to be evaluated for their detrimental effects on the skin. Nowadays, the therapeutic potential of stem cells has and fibroblasts have increased the hope for a successful cell therapy in aesthetic medicine. Stem cells are unspecialized cells capable of renewing themselves through cell ...

full text

reflections on taught courses of the iranian ma program in english translation: a mixed-methods study

the issue of curriculum and syllabus evaluation and revision has been in center of attention right from when curriculum came into attention of educational institutions. thus everywhere in the world in educational institutions curricula and syllabi are evaluated and revised based on the goals, the needs, existing content, etc.. in iran any curriculum is designed in a committee of specialists and...

a comparative study of the theme of "marriage" in three victorian novelists: charles dickens, george eliot and thomas hardy

این تحقیق کوشیده است با انتخاب پنج رمان از میان آثار برگزیده، «چارلز دیکنز»، «جورج الیوت» و «توماس هاردی » و با رویکردی تطبیقی به بررسی درونمایه ی «ازدواج» در آثار این سه رمان نویس بزرگ دوره ی ویکتوریا بپردازد. فرضیه ی اولیه ی تحقیق این بوده است که چارلز دیکنز (1870- 1812) با زبانی شوخ و گزنده، جورج الیوت(1880- 1819) با نگاهی آموزشی–اندرزگونه و توماس هاردی(1928- 1840) با رویکردی تلخ وانتقادی به...

role of cultured skin fibroblasts in aesthetic and plastic surgery

the skin is the largest tissue of man with several functions. cosmetics/cosmeceuticals agents always are needed to be evaluated for their detrimental effects on the skin. nowadays, the therapeutic potential of stem cells has and fibroblasts have increased the hope for a successful cell therapy in aesthetic medicine. stem cells are unspecialized cells capable of renewing themselves through cell ...

full text

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 7  issue None

pages  204- 211

publication date 2018-04

By following a journal you will be notified via email when a new issue of this journal is published.

Keywords

No Keywords

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023