Assessment of some phenolic acids of artichoke callus under in vitro conditions. Mahsa Sanavi Joshaghani*, Azim Ghasemnezhad, Mahdi Alizadeh and Atana Tanuri

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Abstract:

Medicinal plants are the most important sources of life saving drugs for the majority of world's population. Artichoke is one of the oldest medicinal plants from asteraceae family. In the present study, caffeic acid and chlorogenic acid accumulation of different organs of artichoke callus were studied under in vitro conditions in MS, SH, and B5 media. The experiment was performed based on a completely randomized design with 3 replications. The artichoke seeds were transferred to the sterile Petri dishes containing filter paper immediately after disinfecting under sterile conditions. The obtained seedlings were transferred to half-strength MS medium.  Only non-contaminated seedlings were used for preparation of root, leaf, and petiole explants. The SH, MS, and B5 media containing 0.8% agar, 3% sucrose, and 0, 0.5, 0.75, and 1 (mg/l) 2,4-D were used for callus induction. According to the results, caffeic acid accumulation was not found to be statistically different (α

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Journal title

volume 4  issue 4

pages  1151- 1158

publication date 2014-08-01

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