Application of microsatellite DNA method in determining genetic diversity of farmed broodstocks of Penaeus vannamei (Boone, 1931) in hatchery centers of Bushehr province

Authors

Abstract:

The aim of this study was to detect different populations of Pacific White Shrimp (Penaeus vannamei: Boone, 1931) in farmed broodstocks and to determine their genetics indeces in hatchery centers of Bushehr province. First, the origin of the farmed broodstocks was determined based on the historical information of the different stocks. Then, DNA was extracted from muscle tissue of 30 broodstocks from 11 different stocks using a commercial kit. The repeated sequences were amplified using 10 specific primers by PCR in Iranian Shrimp Center. The results showed that the number of alleles was in range of 4.5-5.5 in studied stocks. The most allele frequency was observed in the farmed broadstocks of the third stock in H2 hatchery center, 5.2±0/359. Also, the average of observed heterozygosity in the farmed broadstocks of the second stock in H5 hatchery center obtained 0.669±0.152 that was more than other stocks among all centers. The results of the present study showed that genetics indeces of of third stock in H2 hatchery center were increased compared to other stocks in Bucher province. The inbreeding coefficients of the first stocks in H1 (0.595±0.105) and H3 (0.547±0.145) hatchery centers were significantly higher than other stocks. The inbreeding coefficient increase in farmed broadstocks in these stocks could be related to the small size of founder population and the bias selection programs. However, according to the information from other hatchery centers in Bushehr province and other countries, this amount is acceptable. According to the obtained results, it can be concluded that to increase the genetic indicators in the centers, breeders with higher diversity should be used.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

Genetic characteristics of different populations of Litopenaeus vannamei in hatchery centers of Bushehr province

The aim of this study was identifying different populations and determining the genetic characteristics of three different sub-adult stocks of Litopenaeus vannamei in hatchery of Iran Shrimp Research Center by microsatellites DNA markers in Bushehr province during from October to November 2019. Up to 50 individuals were sampled per stock, with equal contribution by sex. The tissue preserved in ...

full text

phylogeography and genetic diversity of the lesser mouse- eared bat (myotis blythii) in iran

in current study, 63 samples of bat populations collected from differ regions were used for evaluating the geographic variations. twenty cranial and dental characters for traditional morphometric and landmarks method on the ventral, dorsal skull and mandible for geometry morphometric studies were used. statistical analyses of traditional morphometric and geometry morphometric data indicated low...

morphology, geochemistry, mineralogy, and micromorphology of soils of hormozgan province in relation to parent materials

ویژگی های زمین شیمیایی، کانی شناسی، و میکرومورفولوژیکی خاک ها و سنگ مادر مربوطه در منطقه بین بخش های جنوبی زاگرس و خلیج فارس تا دریای عمان(استان هرمزگان، ایران) مورد بررسی قرار گرفت. هدف های این مطالعه شناسایی تغییرات در خصوصیات فیزیکی، شیمیایی، و ترکیب کانی شناسی خاک، مطالعه میکرومورفولوژی و تکامل خاک، و بررسی توزیع عنصر خاک بر اساس هوازدگی، پروسه های خاک و زمین شناسی جهت توصیف اثرات مواد مادر...

15 صفحه اول

application of brand personality scale in automobile industry: the study of samand’s brand personality dimensions

این تحقیق شخصیت برند سمند را در ایران با استفاده از مدل پنج بعدی آکر (1997) بعنوان یک چهارچوب بطور توصیفی سنجیده است. بنابر این چهارچوب که دراصل در 42 جزء (42 ویزگی شخصیتی) ودر پنج بعد شخصیتی طراحی شده بود ودر کشورها وصنایع مختلف آزموده شده بود, پرسنامه به زبان فارسی ترجمه شده و با استفاده از روشهای ترجمه معکوس و مصاحبه عمیق با 12 متخصص ایرانی به 38 جزء کاهش یافت. و نظرسنجی ای در پنج نمایندگی ا...

15 صفحه اول

the role of application of dynamic assessment approach in improvement of iranian efl writing performance at different language proficiency levels

the present study sought to investigate the role of dynamic assessment (da) in improvement of iranian efl writing performance at different language proficiency levels. to this end, after conducting the quick placement test, 60 iranian efl learners were assigned to two groups with different language proficiency levels. in both groups each participant wrote two compositions, one before and one af...

faculty of psychology and social sciences group of anthropology master thesis in major of anthropology

چکیده پایان نامه (شامل خلاصه، اهداف، روش های اجرا و نتایج به دست آمده): کار جمع آوری گو یش های محلی در سال های اخیر شتاب امیدوار کننده ای به خود گرفته است. شاید از بارزترین اهداف جمع آوری گویش های مختلف، ثبت و ضبط آن، جلوگیری از نابودی و مهمتر از همه حل مشکلات دستوری زبان رسمی باشد. دقت در فرآیند های زبانی گویش های محلی نوع ارتباط مردم نواحی مختلف با پیرامون نشان را به ما نشان خواهد داد. از س...

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 12  issue 3

pages  72- 82

publication date 2023-03

By following a journal you will be notified via email when a new issue of this journal is published.

Keywords

No Keywords

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023