Acute application of cholecystokinin and its effect on long-term potentiation induction at CA1 area of hippocampal formation in rat

Authors

  • Farzane Dehghani Applied Physiology Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
  • Parham Reisi Applied Physiology Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
Abstract:

Introduction: It has been demonstrated that cholecystokinin sulfated octapeptide (CCK-8s) can affect synaptic transmission in the hippocampus. Because one of the major experimental models to understand the events happening in synaptic plasticity is To Study the long-term potentiation (LTP), we decided to investigate the effect of concomitant administration of CCK-8s and tetanic stimulation of Schaffer collateral path-CA1 synapses on LTP induction and maintenance.&nbsp;Materials and Methods: Experimental groups were control, CCK-5min and CCK-30min. CCK-8s was injected 5 or 30 min (1.6 &mu;g/kg; i.p.) prior to induction of LTP. The stimulating and the recording electrodes were placed in the Schaffer collateral pathway and hippocampal CA1, respectively. LTP was induced by 100 Hz tetanization and field excitatory postsynaptic potentials (fEPSP) slope, area and amplitude were measured and compared during 30 minutes Interval before, and 90 minutes Interval after LTP induction in each group.&nbsp;Results: The results showed that maintenance of the induced LTP was significantly improved in the CCK-30min group comparing to the control group. This improvement was particularly visible in the fEPSP slope (p<0.001) and the fEPSP area (p<0.001). Seventy minutes after the LTP induction, fEPSP was similar in both the CCK-5min and the CCK-30min groups and there was Also a significant difference between the treated groups comparing to the control group (p<0.05).&nbsp;Conclusion: These results indicated that LTP induction and maintenance is carried out effectively, at higher levels of CCK in the brain. The data suggest that CCK-8s has pronounced effects on synaptic plasticity in the hippocampus and the consequent cognitive functions.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

acute application of cholecystokinin and its effect on long-term potentiation induction at ca1 area of hippocampal formation in rat

introduction: it has been demonstrated that cholecystokinin sulfated octapeptide (cck-8s) can affect synaptic transmission in the hippocampus. because one of the major experimental models to understand the events happening in synaptic plasticity is to study the long-term potentiation (ltp), we decided to investigate the effect of concomitant administration of cck-8s and tetanic stimulation of s...

full text

The effects of CCK-8S on spatial memory and long-term potentiation at CA1 during induction of stress in rats

Objective(s): Cholecystokinin (CCK) has been proposed as a mediator in stress. However, it is still not fully documented what are its effects. We aimed to evaluate the effects of systemic administration of CCK exactly before induction of stress on spatial memory and synaptic plasticity at CA1 in rats. Materials and Methods: Male Wistar rats were divided into 4 groups: the control, the control-C...

full text

The Effect of Noise Pollution Exposure during Pregnancy on Long Term Potentiation Induction in Pyramidal Neurons of Hippocampus CA1 area in Male Rat Offsprings

Background: It is believed that cognitive processing is easily disturbed by incompatible environmental stimulations. Many studies have shown that prenatal stress affects fetal brain development. The aim of this study was to evaluate the effect of noise pollution exposure during conception period on neural activity of hippocampus CA1 area in male rat offspring. Methods: Four groups of rats inclu...

full text

The effect of cysteamine on long-term potentiation and paired-pulse stimulation in the CA1 region of hippocampal slices in rat

The effect of cysteamine, a somatostatin depletor, on synaptic plasticity induced by tetanic and paired-pulse stimulation was investigated in rat hippocampal CA1. For this purpose, hippocampal slices from saline (1 ml/kg, s.c.) and cysteamine (200 fig/kg, s.c.)-treated and intact rats were used. Population spikes were recorded following Schaffer collateral stimulation. To induce LTP, primed bur...

full text

the effects of keyword and context methods on pronunciation and receptive/ productive vocabulary of low-intermediate iranian efl learners: short-term and long-term memory in focus

از گذشته تا کنون، تحقیقات بسیاری صورت گرفته است که همگی به گونه ای بر مثمر ثمر بودن استفاده از استراتژی های یادگیری لغت در یک زبان بیگانه اذعان داشته اند. این تحقیق به بررسی تاثیر دو روش مختلف آموزش واژگان انگلیسی (کلیدی و بافتی) بر تلفظ و دانش لغوی فراگیران ایرانی زیر متوسط زبان انگلیسی و بر ماندگاری آن در حافظه می پردازد. به این منظور، تعداد شصت نفر از زبان آموزان ایرانی هشت تا چهارده ساله با...

15 صفحه اول

Effect of Vitamin D3 on Improvement of Learning and Spatial Memory Following Demyelination induction in hippocampal CA1 Area of rat

Introduction: Consumption of vitamin D3 is effective to reduce intensity of autoimmune diseases such as multiple sclerosis. Neurons of the central nervous system are constantly exposed to reactive oxygen species and these factors play a key role in the destruction of myelin and damage of axons. The hippocampus is a vital center for learning and memory in central nervous system. This area is ...

full text

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 19  issue None

pages  241- 246

publication date 2015-12

By following a journal you will be notified via email when a new issue of this journal is published.

Keywords

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023