A protocol and detailed methodological study on immunogenicity of various experimental vaccines.

This study explores the methodology of various experimental vaccines and their immunogenicity outcome in detail. The idea is to give to the reader a picture of the research methodology behind vaccine development that was used in this research. The novelty of this study bases on the demonstration of the cause and effect of antibodies produced against bacterial and viral proteins (immunogens) as idiotypic-antiidiotypic interactions that can be manipulated to bring a desired outcome. Furthermore, this preclinical study in vaccinology can be the root for building clinical trials of vaccines against important infectious microorganisms affecting humans. Methodological aspects of three experimental vaccines were shown. These vaccines were anti-HIV, anti-Salmonella, and anti-Staphylococcus aureus. The author concludes that eggs from chickens immunized with specific immunogen might be considered as a special type of oral anti-idiotypic vaccines. It was shown by development of anti-anti-idiotypic antibodies that were developed against various epitopes.