A proposal for the unification of two cyanobacterial strains of Nostoc as the same species

Authors

  • David Fewer Prof., Division of Microbiology and Biotechnology, Department of Food and Environmental Sciences, University of Helsinki, Finland
  • Karina Sivonen Prof., Division of Microbiology and Biotechnology, Department of Food and Environmental Sciences, University of Helsinki, Finland
  • Ramezan-Ali Khavari-Nejad Prof., Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran., Faculty of Biological Sciences, Kharazmi University, Tehran, Iran
  • Taher Nejadsattari Associate Prof., Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
Abstract:

The present research aimed to investigate through a polyphasic approach, the differences in morphological and genotypic features of two cyanobacterial strains isolated from paddy fields of northern parts of Iran, belonging to the family Nostocaceae (subsect. IV. I). Based on the reliable identification keys, the two strains were identified as Nostoc ellipsosporum (Desm.) Rabenh. ex Born. et Flah, and N. muscorum Ag. ex Born. et Flah. Sequencing of cloned bacterial 16S rRNA fragment strongly indicated that, two Nostoc strains could not be discriminated by 16S rRNA and ITS genes sequencing and the current morphological classification of both is invalid. Moreover, phylogenetic study of two Nostoc strains has demonstrated that, genetic relationships are in conflict with the morphological classification. Besides, after doing DNA-DNA reassociation experiments, it was concluded that, two Nostoc strains investigated might possibly be united into one species. Finally, two Nostoc species were named as Nostoc sp. Bahar_E and Nostoc sp. Bahar_M, and have been registered under DDBJ, accession numbers: JF795278 and JF272482, respectively.

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Journal title

volume 17  issue 2

pages  161- 172

publication date 2017-02-19

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