مقایسه درصد حیات و تمایز خود به خودی سلول‌های بنیادی بافت چربی و سلول‌های استرومایی مغز استخوان در پاساژهای طولانی مدت

Background and Objective: Adipose tissue-derived stem cells (ADSCs) are very similar in their cell properties to bone marrow stromal cells (BMSCs) and can be a suitable alternative for cell therapy trials. The purpose of this study is comparison of adipose-derived stem cells (ADSCs) and bone marrow stromal cells (BMSCs) in prolonged passages based on viability and auto-differentiation toward adipocytes and osteocytes, respectively. Materials and Methods: In this experimental study, stem cells were harvested from adipose tissue and bone marrow, then, at different cell passages the percentage of dead cells was assessed by live and dead cells assay and spontaneous differentiation toward adipocytes and osteocytes evaluated using Oil Red O and Alizarin red staining, respectively. T-test and one-way ANOVA statistical analysis was used for assessment of obtained data. Results: Attained results of this study showed that the mean percentage of death cells during 5th, 10th and 15th for BMSCs were (2.26±0.36, 3.39±0.45, 6.92±0.95) and for ADSCs were (0.7±0.19, 0.89±0.39, 3.23±0.55), respectively. Also spontaneous differentiation into bone-forming cells of ADSCs and BMSCs in 15th passage shown significant difference for BMSCs (3.25± 0.38) and ADSCs (0.48±0.3) (p< 0.05). Our data show that ADSCs did not spontaneously adipogenic differentiate during 15th passage, but adipogenic differentiation for BMSCs was positive (1.07±0.24). Conclusion: Considering of the percentage of dead cells and spontaneous differentiation with long passages revealed that ADSCs can be a suitable alternative source for BMSCs in therapeutic applications. Key words: Adipose tissue-derived stem cells, Bone marrow stromal cells, Spontaneous differentiation, Survival cells   Funding: This research was funded by Ardabil branch, Islamic Azad University of Medical Sciences. Conflict of interest: None declared. Ethical approval: The Ethics Committee of Tarbiat Modarres University of Medical Sciences approved the study.   How to cite this article: Abdanipour A.R, Noori-Zadeh A, Mohamadi Z, Rashid Sheykh Ahmad F, Akbari P .Comparison of Adipose-Derived Stem Cells (ADSCs) and Bone Marrow Stromal Cells (BMSCs) in Prolonged Passages Based on Viability and Auto-Differentiation. J RafsanjanUniv Med Sci 2015 13(12): 1141-52. [Farsi]