فعالیت ضد باکتریایی عصاره‌متانولی و استونی گال خرنوک درخت بلوط علیه باکتری‌های گرم مثبت

Background: Quercus infectoria gall (Oak) has a long history of use as a medicinal plant. Andricus moreae galls arise on young branches of Quercus infectoria as a result of attack by bees.In this research, the antibacterial activity of methanol and acetone extracts of gall (Quercus infectoria) against Staphylococcus aureus, Bacillus cereus, B.subtilis strains was evaluated. Material and methods: this empirical-experimental study was carried out in Autumn,2011. Getlls were collected from Lorestan oak forests and then were dried and grinded. Acetone and methanol extracts of the galls were prepared by maceration. The viscosities of 50,25,12.5,6.25,3.12 and 1.56 mg/ml were provided from methanol and aceton extracs by serial dilution method. The agar plate well diffusion method was used for antibacterial assay of different samples. Broth microdillution method was used for evaluate MIC and MBC of extracts. Data was analyzed by SPSS-16 software. Results: All extracts demonstrated significant inhibitory effect against selected bacterial strains. There were statistically significant correlations between antibacterial activity and extracts concentration (p<0.05). Antibacterial activity of Andricus moreae gall methanol and acetone extracts against Staphylococcus aureus was more than Bacillus cereus, B.subtilis. The MIC values of the Andricus moreae gall extracts ranged from 1.56 mg/ml to 3.13 mg/ml whereas the MBC values ranged from 3.13mg/ml to 6.25 mg/ml. Conclusion: Methanol and acetone extracts of Andricus moreae were effective against all of tested bacteria. These findings show that methanol and acetone extracts of galls of Quercus infectoria may can be suggested as a natural antibacterial treatment .